74644-66-9Relevant articles and documents
Methenyltetrahydrofolate cyclohydrolase catalyzes the synthesis of (6S)-5-formyltetrahydrofolate
Pelletier, Joelle N.,Mackenzie, Robert E.
, p. 220 - 228 (1996)
The methenyltetrahydrofolate cyclohydrolase activity of the bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase domain catalyzes the conversion of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. We have observed that in the presence of this domain, 10-formyltetrahydrofolate is converted to 5-formyltetrahydrofolate. The effects of nucleotide analogs on the initial rates of this reaction were found to be similar to their effects on the cyclohydrolase activity, establishing that 5-formyltetrahydrofolate production is dependent on the cyclohydrolase. The specific activity of 5-formyltetrahydrofolate production is approximately 7 x 104-fold lower than that of the cyclohydrolase activity but can be used to obtain quantitative conversion of 10-formylH4folate to 5-formyltetrahydrofolate, in mg amounts. This 'side-reaction' may contribute to the in vivo production of (6S)-5-formyltetrahydrofolate.
