80498-17-5

Basic information

• Product Name: ECOR I
• Synonyms: ecorirestrictionendonuclease;endodeoxyribonucleaseecori;e.c.3.1.23.13;Einecs 279-487-9;restrictionendodeoxyribo-nucleasecori;restrictionendonucleaseecori;restrictionendonucleasessoi;restriction endonuclease ecor I from*escherichia
• CAS NO: 80498-17-5
• Molecular Weight: 0
• EINECS: 279-487-9
• Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
• Mol File: 80498-17-5.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: ECOR I(CAS DataBase Reference)
• NIST Chemistry Reference: ECOR I(80498-17-5)
• EPA Substance Registry System: ECOR I(80498-17-5)

Safety Data

• WGK Germany: 3
• RTECS: RF0757500
• Hazardous Substances Data: 80498-17-5(Hazardous Substances Data)

Usage

Description

ECOR I, also known as EcoRI, is a type II restriction endonuclease enzyme derived from the bacterium Escherichia coli RY13. It is widely used in molecular biology for its ability to recognize and cleave specific DNA sequences, making it a valuable tool for various genetic engineering techniques.
Used in Molecular Biology:
ECOR I is used as a restriction endonuclease for cutting DNA at the recognition site 5′-G/AATTC-3′, which allows for the generation of DNA fragments with 5′-cohesive termini. This property is essential for various molecular biology applications, such as cloning, gene expression studies, and DNA fingerprinting.
Used in Genetic Engineering:
In the field of genetic engineering, ECOR I is used as a tool for DNA manipulation. Its ability to create specific cuts in DNA sequences enables scientists to insert, delete, or modify genes within an organism's genome, facilitating the development of genetically modified organisms and the study of gene function.
Used in DNA Cloning:
ECOR I is used as a restriction enzyme in DNA cloning processes. By recognizing and cutting DNA at specific sites, it allows researchers to insert foreign DNA sequences into a host organism's genome, creating recombinant DNA molecules that can be used for various purposes, such as producing proteins of interest or studying gene regulation.
Used in DNA Fingerprinting:
ECOR I is used as a tool for DNA fingerprinting, a technique used to identify individuals based on their unique DNA sequences. By cutting DNA at specific recognition sites, it helps create a pattern of DNA fragments that can be used to distinguish between individuals and establish genetic relationships.
Used in Gene Expression Studies:
In gene expression studies, ECOR I is used to analyze the activity of specific genes. By cutting DNA at the recognition site, it allows researchers to isolate and study gene fragments, providing insights into gene regulation and expression patterns under various conditions.
Overall, ECOR I is a versatile enzyme with numerous applications in molecular biology, genetic engineering, DNA cloning, DNA fingerprinting, and gene expression studies, making it an indispensable tool for researchers in the field of genetics and molecular biology.