87081-35-4 Usage
Description
Leptomycin B from Streptomyces sp is a nanomolar active and specific nuclear export inhibitor, isolated from selected Streptomyces strains. It is the dominant and most studied member of the leptomycin class, exhibiting antifungal, antibacterial, and potent antitumor activities. Leptomycin B targets CRM1/exportin1, a protein in the nuclear export sequence (NES), and affects various proteins, including c-Abl, cyclin B1, HIV-1 Rev, IκB, MPF, MAP/ERK, MDM2/p53, NF-κB/IκB7, and PKA. It inhibits the export of many RNAs, such as COX-2 and c-FOS mRNA.
Uses
Used in Pharmaceutical Industry:
Leptomycin B from Streptomyces sp is used as an anti-fungal antibiotic and anti-tumor cytotoxin for its potent antitumor activities and ability to inhibit CRM1-dependent, NES-dependent nucleo-cytoplasmic translocation.
Used as a Biological Tool in Research:
Leptomycin B from Streptomyces sp is used as a biological tool for studying nuclear localization and protein trafficking in eukaryotic cells, as well as a potent, specific inhibitor of nuclear export signal (NES)-dependent protein export from the nucleus.
Used in Drug Development:
Leptomycin B from Streptomyces sp is used in drug development for its potential applications in stabilizing p53 by blocking its nuclear export, which can have implications in cancer treatment and therapy.
Biological Activity
Antifungal antibiotic that is an inhibitor of the nuclear export of proteins; acts by binding directly to and inhibiting CRM1/exportin-1. Inhibits the nuclear export of the HIV regulatory protein Rev and stabilizes p53. Antitumor in vitro and in vivo .
Biochem/physiol Actions
Leptomycin B is an unsaturated, branched-chain fatty acid, and is an important tool in the study of nuclear export. Leptomycin B is a specific inhibitor of proteins containing nuclear export signal. Leptomycin B inhibits nucleo-cytoplasmic translocation of molecules such as the HIV-1 Rev protein and Rev-dependent export of mRNA. The addition of very small amounts to fibroblasts causes accumulation of MEK in the nucleus. Other proteins that are influenced by leptomycin?B are actin, c-Abl, cyclin B1, MDM2/p53, IκB, MPF, and PKA. The suggested inhibition mechanism involves the direct binding of leptomycin?B to CRM1, which blocks the binding of CRM1 to proteins containing the nuclear export signal, via the interaction with cysteine residue in CRM1 control conserved region.
References
1) Kudo et al. (1998), Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1; Exp. Cell Res., 242 540
2) Wolff et al. (1997), Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA; Chem. Biol., 4 139
3) Freedman and Levine (1998), Nuclear export is required for degradation of endogenous p53 by MDM2 and human papillomavirus E6; Mol. Cell. Biol., 18 7288
Check Digit Verification of cas no
The CAS Registry Mumber 87081-35-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,7,0,8 and 1 respectively; the second part has 2 digits, 3 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 87081-35:
(7*8)+(6*7)+(5*0)+(4*8)+(3*1)+(2*3)+(1*5)=144
144 % 10 = 4
So 87081-35-4 is a valid CAS Registry Number.
InChI:InChI=1/C33H48O6/c1-9-28(14-15-29-24(5)13-16-31(36)39-29)19-22(3)12-10-11-21(2)17-25(6)32(37)27(8)33(38)26(7)18-23(4)20-30(34)35/h10-11,13-17,19-20,22,24-27,29,33,38H,9,12,18H2,1-8H3,(H,34,35)/b11-10+,15-14+,21-17+,23-20+,28-19-/t22-,24+,25-,26+,27-,29+,33-/m1/s1
87081-35-4Relevant articles and documents
Absolute stereostructure and total synthesis of leptomycin B
Kobayashi, Motomasa,Wang, Weiqi,Tsutsui, Yasuhiro,Sugimoto, Masanori,Murakami, Nobutoshi
, p. 8291 - 8294 (1998)
The absolute stereostructure of leptomycin B, an antitumor antibiotic and inhibitor of nuclear protein export, was firstly presumed as 1 having 4S, 5R, 10R, 16R, 18S, 19R, 20S on the basis of NMR comparison with callystatin A (2) and then 1 was asymmetric