9012-36-6

Basic information

• Product Name: AGAROSE
• Synonyms: QA-AGAROSE(TM);QA-AGAROSE(TM) LOW MELTING;QA-AGAROSE(TM) MULTIPURPOSE;MULTI-PURPOSE AGAROSE;MOLECULAR-SCREENING AGAROSE;SERVA AGAROSE;SEPHAROSE(R) 2B;SEPHAROSE(R) 4B
• CAS NO: 9012-36-6
• Molecular Formula: C10H15N3O3
• Molecular Weight: 0
• EINECS: 232-731-8
• Product Categories: Gel Filtration Chromatography;Gel Filtration Media;Gel Filtration MediaResins&LC Separation Media;Protein Chromatography;Sepharose;gel
• Mol File: 9012-36-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: ≤90 °C (4% in water)(lit.)
• Boiling Point: 100℃
• Flash Point: 38 °C
• Appearance: White to slightly cream/suspension (75% in water)
• Density: 1.00 g/mL at 20 °C
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: 1.679
• Storage Temp.: 2-8°C
• Solubility: H2O: 20 mg/mL hot, slightly hazy, very faintly yellow
• Water Solubility: Soluble in water.
• Sensitive: Moisture Sensitive & Hygroscopic
• Stability: Stable. Incompatible with strong acids, strong oxidizing agents.
• CAS DataBase Reference: AGAROSE(CAS DataBase Reference)
• NIST Chemistry Reference: AGAROSE(9012-36-6)
• EPA Substance Registry System: AGAROSE(9012-36-6)

Safety Data

• Hazard Codes: Xi
• Statements: 10-36/37/38
• Safety Statements: 16-26-36
• RIDADR: NA 1993 / PGIII
• WGK Germany: 3
• F: 3
• TSCA: Yes
• HazardClass: 3
• Hazardous Substances Data: 9012-36-6(Hazardous Substances Data)

Usage

Description

Agarose is a gelatinous, non-nutritive polysaccharide derived from red seaweed, composed of alternating agarobiose units. It is known for its unique properties, such as forming gels upon cooling and melting upon heating, without any change in its chemical structure. These characteristics make agarose a versatile substance in various applications.
Used in Molecular Biology:
Agarose is used as a gel matrix for the separation of large molecules, particularly DNA, by electrophoresis. It is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Agarose gels provide low ethidium bromide and SYBR Green background staining.
Used in High Resolution Protein Electrophoresis:
Agarose is used as a gel matrix for high-resolution protein electrophoresis, immunoelectrophoresis, electro immunoassays such as rocket assays, and counter current electrophoresis. It offers a wide range of different agaroses, of varying molecular weights and properties, which are commercially available for these purposes.
Used in Separation of Small DNA Fragments:
High-resolution agarose is used for the separation of small DNA fragments, such as PCR products, differing in size by as little as 2%. It provides a resolution comparable to that of DNA in polyacrylamide gels and is ideal for resolving amplification fragment length polymorphisms (AmpFLP), short tandem repeats (STR), and triand tetranucleotide repeats. High-resolution agarose also offers low ethidium bromide and SYBR Green background staining.

Biochem/physiol Actions

Agarose is used as bioink, because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

InChI:InChI=1/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1