Detection of Menadione sodium bisulfite (cas 130-37-0) (vitamin K 3 ) by reversed-phase high performance liquid chromatography with series dual-electrode amperometric detector
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Add time:09/29/2019 Source:infona.pl
A reversed-phase high-performance liquid chromatography with series dual glassy carbon electrodes for the amperometric detection of water-soluble menadione is described. The complex post-column derivatization reaction and the high background currents were avoided. The Menadione sodium bisulfite (cas 130-37-0) was reduced at -0.3 V vs. SCE at the upstream (generator) electrode and oxidized at +0.2 V vs. SCE at the downstream (collector) electrode. The mobile phase was 0.2 mol l - 1 HAc-NaAc aqueous buffer (pH 5.50) and 40% (v/v) methanol. The linear response was in the range of 35 ng to 15 μg, with a detection limit of 15 ng (S/N=3). The correlation coefficient was 0.9997 (n=6). The electrochemical detection with series dual electrodes has a higher selectivity for menadione (vitamin K 3 ) compound than with UV detection.
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