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Biocytin

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Name

Biocytin

EINECS N/A
CAS No. 576-19-2 Density 1.234g/cm3
PSA 158.85000 LogP 2.15940
Solubility N/A Melting Point 241-243 °C
Formula C16H28N4O4S Boiling Point 748 °C at 760 mmHg
Molecular Weight 372.489 Flash Point 406.2 °C
Transport Information N/A Appearance White Solid
Safety Risk Codes N/A
Molecular Structure Molecular Structure of 576-19-2 (Biocytin) Hazard Symbols N/A
Synonyms

L-Lysine, N~6~-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-;N~6~-{5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}-L-lysine;L-Lysine, N6-(5-(hexahydro-2-oxo-1H-thieno(3,4-d)imidazol-4-yl)-1-oxopentyl)-, (3aS-(3aalpha,4beta,6aalpha))-;N-EPSILON-D-BIOTINYL-L-LYSINE;Biotinyl-L-lysine;L-BIOCYTIN;

Article Data 12

Biocytin Synthetic route

N-(4-((S)-5'-oxo-9λ4-boraspiro[bicyclo[3.3.1]nonane-9,2'-[1,3,2]oxazaborolidin]-4'-yl)butyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

576-19-2

biocytin

Conditions
ConditionsYield
With tetrabutyl ammonium fluoride In tetrahydrofuran; water at 20℃; for 2h;73%
56-87-1

L-lysine

91853-90-6

biotin chloride

576-19-2

biocytin

Conditions
ConditionsYield
With sodium hydroxide; chloroform
857358-40-8

N2-formyl-N6-[5-((3aS)-2-oxo-(3ar,6ac)-hexahydro-thieno[3,4-d]imidazol-4t-yl)-valeryl]-L-lysine

576-19-2

biocytin

Conditions
ConditionsYield
With hydrogenchloride
608-16-2

biotin methyl ester

576-19-2

biocytin

Conditions
ConditionsYield
With toluene; 1,2,4-Trichlorobenzene
62062-43-5

Boc-Lys(biotinyl)-OH

576-19-2

biocytin

Conditions
ConditionsYield
With methoxybenzene; trifluoroacetic acid
56-87-1

L-lysine

biotin-ethyl ester

biotin-ethyl ester

576-19-2

biocytin

Conditions
ConditionsYield
With toluene; 1,2,4-Trichlorobenzene
35013-72-0

biotin N-Hydroxysuccinimide ester

576-19-2

biocytin

Conditions
ConditionsYield
Stage #1: biotin N-Hydroxysuccinimide ester With sodium hydrogencarbonate In water; N,N-dimethyl-formamide
Stage #2: With ethylenediaminetetraacetic acid In water
Multi-step reaction with 2 steps
1: sodium hydroxide / 1,4-dioxane; water / 20 °C / pH 8 - 8.5
2: 1,8-diazabicyclo[5.4.0]undec-7-ene / N,N-dimethyl-formamide / 1 h / 20 °C
View Scheme
Multi-step reaction with 2 steps
1: sodium hydroxide / 1,4-dioxane / 22 - 25 °C / pH 8 - 8.5
2: 1,8-diazabicyclo[5.4.0]undec-7-ene / N,N-dimethyl-formamide / 1 h / 22 - 25 °C
View Scheme
19729-28-3

Nα-formyl-L-lysine

576-19-2

biocytin

Conditions
ConditionsYield
Multi-step reaction with 2 steps
1: pyridine
2: aqueous HCl
View Scheme
91853-90-6

biotin chloride

576-19-2

biocytin

Conditions
ConditionsYield
Multi-step reaction with 2 steps
2: toluene; 1,2,4-trichloro-benzene
View Scheme
Multi-step reaction with 2 steps
1: pyridine
2: aqueous HCl
View Scheme
1155-64-2

N6-[(benzyloxy)carbonyl]-L-lysine

576-19-2

biocytin

Conditions
ConditionsYield
Multi-step reaction with 3 steps
1: acetic acid anhydride / und Hydrieren des Reaktionsprodukts an Palladium
2: pyridine
3: aqueous HCl
View Scheme

Biocytin Specification

The Biocytin with the cas number 576-19-2, is also called N~6~-{5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}-L-lysine .The product y of this chemical is Biotin Derivatives .It is useful for the synthesis of Biocytin containing peptides to study the mechanism of reactions catalyzed by biotin - containing enzymes.

The properties of the Biocytin are: (1)#H bond acceptors:  8  ; (2)#H bond donors:  6  ; (3)#Freely Rotating Bonds:  12  ; (4)Polar Surface Area:  98.7 Å2  ; (5)Index of Refraction:  1.547  ; (6)Molar Refractivity:  95.8 cm3  ; (7)Molar Volume:  301.7 cm3  ; (8)Polarizability:  37.98×10-24cm; (9)Surface Tension:  50.2 dyne/cm ; (10)Enthalpy of Vaporization:  118.65 kJ/mol ; (11)Vapour Pressure:  1.75×10-24 mmHg at 25°C.

Biological activity of this chemical are: (1)Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays; (2)Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes; (3) Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

This product can be supplied by the following suppliers: (1)3B Pharmachem International (Wuhan) Co.,Ltd.; (2)Nanjing Chemlin Chemical Co., Ltd.; (3)Guangzhou WeiBo Chemical Co., Ltd.; (4)Leap Labchem Co.,Ltd.; (5)Advanced Technology & Industrial Co., Ltd.; (6)MOLEKULA Ltd. ; (7)Toronto Research Chemicals; (8)Medical Isotopes.

You can still convert the following datas into molecular structure :
1.O=C1N[C@@H]2[C@@H](SC[C@@H]2N1)CCCCC(=O)NCCCC[C@@H](C(=O)O)N
2.InChI=1/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1

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