Preparation of a Sulfurtransferase Substrate, Sodium 3-Mercaptopyruvate, from 3-Bromopyruvic Acid and Sodium Hydrosulfide
Sodium 3-mercaptopyruvate (3-MP), which is a sulfur donor substrate for sulfurtransferases, was synthesized directly from 3-bromopyruvic acid in anhydrous methanol at 4 deg.C, by employing sodium hydrosulfide as a sulfhydryl compound instead of hydrogen sulfide gas.The purity of 3-MP was determined to be 97.9 and 98.6 percent by assay with N-ethylmaleimide and with reduced sodium adenine dinucleotide and lactate dehydrogenase, respectively.
Tanabe, Shinzo,Ogasawara, Yuki,Nawata, Masashi,Kawanabe, Koji
p. 2843 - 2845
(2007/10/02)
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