- METHOD OF PRODUCING N-SUBSTITUTED (METH)ACRYLAMIDE
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PROBLEM TO BE SOLVED: To provide a method of producing N-substituted (meth)acrylamide of high purity under mild conditions. SOLUTION: This invention relates to a method of obtaining N-substituted (meth)acrylamide [3] by the detachment of an amine compound through the liquid-phase thermal decomposition of an aminopropionic acid amide derivative [1] in the presence of a catalyst mainly composed of silica, wherein R1-R5 are represented by H, a C1-32 alkyl group, and a hydroxyalkyl group. COPYRIGHT: (C)2016,JPOandINPIT
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Paragraph 0055; 0061; 0062
(2018/12/12)
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- A fluorescent polymeric heparin sensor
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Linear copolymers have been developed which carry binding sites tailored for sulfated sugars. All binding monomers are based on the methacrylamide skeleton and ensure statistical radical copolymerization. They are decorated with o-aminomethylphenylboronates for covalent ester formation and/ or alkylammonium ions for noncovalent Coulomb attraction. Alcohol sidechains maintain a high water solubility; a dansyl monomer was constructed as a fluorescence label. Statistical copoly merization of comonomer mixtures with optimized ratios was started by AIBN (AIBN = 2,2′-azoisobutyronitrile) and furnished water-soluble comonomers with an exceptionally high affinity for glucosaminoglucans. Heparin can be quantitatively detected with an unprecedented 30 nM sensitivity, and a neutral polymer without any ammonium cation is still able to bind the target with almost micromolar affinity. From this unexpected result, we propose a new binding scheme between the boronate and a sulfated ethylene glycol or aminoethanol unit. Although the mechanism of heparin binding involves covalent boronate ester formation, it can be completely reversed by protamine addition, similar to heparin's complex formation with antithrombin III.
- Sun, Wei,Bandmann, Heinz,Schrader, Thomas
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p. 7701 - 7707
(2008/03/14)
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- Tuning linear copolymers into protein-specific hosts
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Being picky: A new concept for selective protein recognition by artificial receptor molecules involves statistical copolymerization of simple binding monomers in defined stoichiometric ratios. As an example of its efficiency, an arginine-rich lysozyme is recognized by a bisphosphonate-rich copolymer with a remarkable Kd value of 25 nM, 100-times superior to that of cytochrome c, which is comparable in size and isoelectric point. (Chemical Equation Presented).
- Koch, Sebastian J.,Renner, Christian,Xie, Xiulan,Schrader, Thomas
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p. 6352 - 6355
(2007/10/03)
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