- Creation of an α-mannosynthase from a broad glycosidase scaffold
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α-Mannosides made easy: Mutation of a family-GH31 α-glucosidase that displays plasticity to alterations at the 2-OH position of donor substrates created an efficient α-mannoside-synthesizing biocatalyst. A simple fluoride donor reagent was used for the synthesis of a range of mono-α-mannosylated conjugates using the α-mannosynthase displaying low (unwanted) oligomerization activity. Copyright
- Yamamoto, Keisuke,Davis, Benjamin G.
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p. 7449 - 7453
(2012/09/21)
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- Galactosylation and glucosylation by use of β-galactosidase
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The transglucosylation activity of β-galactosidase derived from Aspergillus oryzae and Escherichia coli, respectively, was examined in reaction systems containing up to 50% acetonitrile. Starting with ortho-nitrophenyl β-galactoside (1), which functions both as donor and as acceptor, β-Gal(1-6)β-Gal-PhNO2-o (2) and β-Gal(1-3)β-Gal-PhNO2-o (3) were obtained. Under similar conditions the enzyme from A. oryzae converts para-nitrophenyl β-glucoside (5) to β-Glc(1-2)β-Glc-PhNO2-p (6) and α-Glc(1-4)β-Glc-PhNO2-p (7). Incubation of 1 and L-serine in the presence of the A. oryzae β-galactosidase leads to β-Gal-L-Ser (4).
- Sauerbrei,Thiem
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p. 201 - 204
(2007/10/02)
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- A SIMPLE STRATEGY FOR CHANGING THE REGIOSELECTIVITY OF GLYCOSIDASE-CATALYSED FORMATION OF DISACCHARIDES
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The regioselectivity of glycosidase-catalysed formation of disaccharides can be changed by using α- or β-glycosyl acceptors with various aglycons.The preponderant formation of other than (1->6) linkages can be effected with glycosidases which normally give (1->6) linkages.Thus, an α-D-galactosidase can be induced to catalyse the formation mainly of α-(1->2)-, α-(1->3)-, or α-(1->6)-linked digalctosides.Both the structure of the aglycon and the configuration of the glycosidic linkage can have a pronounced influence on the regioselectivity of disaccharide formation.Enzymic syntheses, in yields of 20-30percent, are described of α-D-Galp-(1->3)-α-D-Galp-OMe, β-D-Galp-(1->3)-β-D-Galp-OMe, β-D-Galp-(1->6)-α-D-Galp-OMe, α-D-Manp-(1->2)-α-D-Manp-OMe, α-D-Manp-(1->6)-α-D-Manp-OMe, α-D-Galp-(1->2)-α-D-Galp-OPhNO2-o, α-D-Galp-(1->3)-α-D-Galp-OPhNO2-p, α-D-Manp-(1->2)-α-D-Manp-OPhNO2-p, and α-D-Manp-(1->2)-α-D-Manp-(1->2)-α-D-Manp-OMe.Soluble and immobilised enzymes have been used.
- Nilsson, Kurt G. I.
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- p-NITROPHENYL 2-, AND 3-O-α-D-MANNOPYRANOSYL-α-D-MANNOPYRANOSIDE
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p-Nitrophenyl 3- and 2-O-benzoyl-4,6-O-benzylidene-α-D-mannopyranoside were each condensed with 2,3,4,6-tetra-O-benzoyl-α-D-mannopyranosyl bromide, and the products were deprotected, to yield, respectively, p-nitrophenyl 2- and 3-O-mannopyranosyl-α-D-mann
- Ekborg, Goeran,Glaudemans, Cornelis P. J.
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