The invention belongs to the technical field of chemical aromatic amide compounds, in particular to a calix[4]arene amide compound and a preparation method and application thereof. The preparation method of the calix[4]arene amide compound comprises the following steps of: (1) preparing 5,11,17,23-tetrabromo-25,26,27,28-tetra-n-propyl calix[4]arene (II) in 1,3-alternate conformation; (2) preparing5,11,17,23-tetrathienyl-25,26,27,28-tetra-n-propyl calix[4]arene (III) in 1,3-alternate conformation; (3) preparing 5,11,17,23-tetra(5- formylthiophen)-25,26,27,28-tetra-n-propyl calix[4]arene (IV) in 1,3-alternate conformation; (4) preparing 5,11,17,23-tetra(5-carboxythienyl)-25,26,27,28-tetra-n-propyl calix[4]arene (V) in 1,3-alternate conformation; and (5) dissolving the 5,11,17,23-tetra(5-carboxythienyl)-25,26,27,28-tetra-n-propyl calix[4]arene (V) in 1,3-alternate conformation in 1,2-dichloroethane, cooling the mixture in an ice-water bath, adding thionyl chloride slowly and dropwise, then raising the temperature for reflux for 5 hours, then cooling the obtained mixture in an ice-water bath, adding a mixed solution of ammonia water and methylene chloride for a reaction at room temperature for 8-12 hours to obtain the product 5,11,17,23-tetra(5-acylaminothienyl)-25,26,27,28-tetra-n-propyl calix[4]arene (I) in 1,3-alternate conformation.