- The rid family member RutC of Escherichia coli is a 3-aminoacrylate deaminase
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The Rid protein family (PF14588, IPR006175) is divided into nine subfamilies, of which only the RidA subfamily has been characterized biochemically. RutC, the founding member of one subfamily, is encoded in the pyrimidine utilization (rut) operon that encodes a pathway that allows Escherichia coli to use uracil as a sole nitrogen source. Results reported herein demonstrate that RutC has 3-aminoacrylate deaminase activity and facilitates one of the reactions previously presumed to occur spontaneously in vivo. RutC was active with several enamine-imine substrates, showing similarities and differences in substrate specificity with the canonical member of the Rid superfamily, Salmonella enterica RidA. Under standard laboratory conditions, a Rut pathway lacking RutC generates sufficient nitrogen from uracil for growth of E. coli. These results support a revised model of the Rut pathway and provide evidence that Rid proteins may modulate metabolic fitness, rather than catalyzing essential functions.
- Buckner, Brandi A.,Lato, Ashley M.,Campagna, Shawn R.,Downs, Diana M.
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Read Online
- Carboxamide Compound and Use of the Same
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A carboxamide compound represented by the formula (I): [wherein Q represents a nitrogen-containing 6-membered aromatic heterocyclic group optionally fused with a benzene ring, two or more ring constitutional atoms of the heterocyclic group are a nitrogen atom, and the heterocyclic group may be substituted with at least one group selected from the group consisting of a C1-C3 alkyl group etc.], and a plant disease controlling agent comprising this as an active ingredient.
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Page/Page column 21-22
(2009/05/29)
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- Carboxamide Compound and Use of the Same
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A carboxamide compound represented by the formula (1): [wherein Q represents a nitrogen-containing 6-membered aromatic heterocyclic group optionally fused with a benzene ring, one of ring constitutional atoms of the heterocyclic group is a nitrogen atom and the heterocyclic group may be substituted with a group selected from the group consisting of a C1-C3 alkyl and the like, R1 represents a C1-C3 alkyl group or the like, R2 represents a hydrogen atom or the like, and R3 represents a hydrogen atom or the like] and a plant disease controlling agent comprising this as an active ingredient.
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Page/Page column 15
(2009/05/28)
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- METHODS OF TREATMENT AND PREVENTION OF NEURODEGENERATIVE DISEASES AND DISORDERS
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The present invention provides compostions and methods useful for treating and preventing neurodegenerative disease and neurologically related disorders by inhibition of Lp-PLA2. The compositions and methods are useful for treating and preventing diseases
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- METHODS OF TREATMENT AND PREVENTION OF METABOLIC BONE DISEASES AND DISORDERS
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The present invention provides compostions and methods useful for treating and preventing metabolic bone diseases and disorders by inhibition of Lp-PLA2. The compositions and methods are useful for treating and preventing metabolic bone disease
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- CARBOXAMIDE COMPOUND AND USE THEREOF
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A carboxamide compound represented by the formula (I): [wherein Q represents a nitrogen-containing 5-membered heterocyclic group optionally fused with a benzene ring, R1 represents a C1-C3 alkyl group or the like, R2 represents a hydrogen atom or the like, and R3 represents a hydrogen atom.] has an excellent plant disease controlling effect.
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Page/Page column 32
(2008/06/13)
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- Anti-infective agents
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Compounds having the formula are hepatitis C(HCV) polymerase inhibitors. Also disclosed are a composition and method for inhibiting hepatitis C(HCV) polymerase, processes for making the compounds, and synthetic intermediates employed in the processes.
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- Anti-infective agents
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Compounds having the formula are hepatitis C (HCV) polymerase inhibitors. Also disclosed are a composition and method for inhibiting hepatitis C (HCV) polymerase, processes for making the compounds, and synthetic intermediates employed in the processes.
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- A convenient synthesis of bridged azatricyclic anhydrides
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Bridged N-protected β-amino acids have been regioselectively prepared from the corresponding bicyclic monoesters. The subsequent cyclisation by thionyl chloride produced the desired bridged azatricyclic anhydrides which are versatile substrates for the synthesis of dipeptides; they are also converted into oxathymine and oxauracil by a thermal [4+2] cycloreversion.
- Canonne,Akssira,Dahdouh,Kasmi,Boumzebra
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p. 1985 - 1992
(2007/10/02)
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- SYNTHESE DE β-AMINOACIDES PONTES ET LEUR THERMOLYSE EN DEHYDRO β-AMINOACIDE ET EN OXAURACILE
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Reactions of bridged dicarboxylic anhydride derivatives with sodium azide in THF give the corresponding, N-tert-butyloxycarbonyl β-aminoesters 2(a-c), which can be cyclized to oxanorborno- and norbornouracils 6(a-c).Pyrolysis of these adducts leads to dehydro-β-alanine 5 and oxauracil 7.
- Akssira, M.,Dahdouh, A.,Kasmi, H
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p. 227 - 232
(2007/10/02)
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- 15N NMR Spectroscopy; 24-Chemical Shifts and Coupling Constants of α-Amino Acid N-Carboxyanhydrides and Related Heterocycles
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The chemical shifts of amino acid N-carboxyanhydrides (NCAs) and cyclic or linear urethanes are less sensitive to solvent effects than those of amides and lactams.The values of the one-bond 15N-1H coupling constants depend on the solvent and are 5-8 Hz larger than those of ureas and amides.The 15N-13C coupling constant of the N-CO group is also unusually high, while that of the N-CH group lies within the range known for N-acetylated aliphatic amines.The one-bond 15N-13C coupling constant was found to be insensitive to conformational changes.
- Kricheldorf, Hans R.
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p. 198 - 203
(2007/10/02)
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