- Creation of an α-mannosynthase from a broad glycosidase scaffold
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α-Mannosides made easy: Mutation of a family-GH31 α-glucosidase that displays plasticity to alterations at the 2-OH position of donor substrates created an efficient α-mannoside-synthesizing biocatalyst. A simple fluoride donor reagent was used for the synthesis of a range of mono-α-mannosylated conjugates using the α-mannosynthase displaying low (unwanted) oligomerization activity. Copyright
- Yamamoto, Keisuke,Davis, Benjamin G.
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supporting information; experimental part
p. 7449 - 7453
(2012/09/21)
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- Production of galactinol from sucrose by plant enzymes.
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Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry.
- Wakiuchi, Nariaki,Shiomi, Ryohei,Tamaki, Hajime
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p. 1465 - 1471
(2007/10/03)
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- Syntheses of penta-O-benzyl-myo-inositols, O-β-L-arabinosyl-(1 → 2)sn-myo-inositol, O-α-D-galactosyl-(1 → 3)-sn-myo-inositol, and O-α-D-galactosyl-(1 → 6)-O-α-D-galactosyl-(1 → 3)-sn-myo-inositol
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Two-step conversions of myo-inositol into (±)-2,3,4,5,6- and 1,3,4,5,6-penta-O-benzyl-myo-inositols are described. Starting from these monohydroxy derivatives of myo-inositol, O-β-L-arabinopyranosyl-(1→2)-sn-myo-inositol from Japanese green tea, Camellia sinensis, and O-α-D-galactopyranosyl-(1→3)-sn-myo-inositol (galactinol) as well as its homolog, O-α-D-galactopyranosyl-(1→6(II))-galactinol, were synthesized by way of the in situ activating glycosylation procedure.
- Koto,Hirooka,Yoshida,Takenaka,Nagamitsu,Sakurai,Zen,Yago,Tomonaga
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p. 2521 - 2529
(2007/10/03)
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- DERIVATIVES OF ASYMMETRICALLY SUBSTITUTED MYO-INOSITOL. XXXIII. SYNTHESIS OF DIASTEROMERIC MANNOSIDES OF MYO-INOSITOL
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Diastereomeric acetylated mannosides of myo-inositol were synthesized by the glycosylation of the racemic acetates of myo-inositol with the ethyl orthoacetate of D-mannose.The synthesized mannosides of myo-inositol can be used for the production of chiral myo-inositol acetates and as intermediates in the synthesis of complex phosphoinositides.
- Sibrikov, Yu. I.,Stepanov, A. E.,Shvets, V. I.
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p. 470 - 476
(2007/10/02)
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