- Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy
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Detergents serve as useful tools for membrane protein structural and functional studies. Their amphipathic nature allows detergents to associate with the hydrophobic regions of membrane proteins whilst maintaining the proteins in aqueous solution. However
- Cho, Kyung Ho,Ribeiro, Orquidea,Du, Yang,Tikhonova, Elena,Mortensen, Jonas S.,Markham, Kelsey,Hariharan, Parameswaran,Loland, Claus J.,Guan, Lan,Kobilka, Brian K.,Byrne, Bernadette,Chae, Pil Seok
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- NOVEL MESITYLENE-CORED AMPHIPHILES AND USES THEREOF
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The present invention relates to a mesitylene-cored amphiphile, a method of preparing the same and a method of extraction, solubilization, stabilization, crystallization or analysis of a membrane protein using the same. When a mesitylene-cored compound according to the present invention is used, membrane proteins can be stored in a stable conformation for a long time in an aqueous solution in comparison with conventional compounds, by which the membrane proteins are usable in both functional and structural analyses thereof. The functional and structural analyses of membrane proteins are one of the fields that are most spotlighted in current biology and chemistry. The present invention can be used to research on a protein structure closely related to new drug development.
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Paragraph 126; 127; 137; 138; 148; 149
(2018/02/28)
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- NOVEL XYLENE-BASED AMPHIPHILIC COMPOUND AND USE THEREOF
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The present invention relates to a xylene-based amphiphilic compound, a method for preparing the same, and a method for extracting, solubilizing, stabilizing or crystallizing a membrane protein using the same. By using the xylene-based compound according to the present invention, a membrane protein may be stably stored in an aqueous solution for a long time, and may be applied in functional and structural analyses. The structural and functional analysis of the membrane protein is one of the fields of highest interest in biology and chemistry, and the xylene-based compound according to the present invention can be applied in research on protein structure that is closely related to development of a new drug.
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Paragraph 0168; 0178; 0179; 0188; 0203
(2018/10/19)
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- Novel Xylene-Linked Maltoside Amphiphiles (XMAs) for Membrane Protein Stabilisation
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Membrane proteins are key functional players in biological systems. These biomacromolecules contain both hydrophilic and hydrophobic regions and thus amphipathic molecules are necessary to extract membrane proteins from their native lipid environments and
- Cho, Kyung Ho,Du, Yang,Scull, Nicola J.,Hariharan, Parameswaran,Gotfryd, Kamil,Loland, Claus J.,Guan, Lan,Byrne, Bernadette,Kobilka, Brian K.,Chae, Pil Seok
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supporting information
p. 10008 - 10013
(2015/07/07)
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