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475-25-2

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Hummel,Perkin,A. G.
(1882)
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Oxidation pathways of natural dye hematoxylin in aqueous solution
The oxidation mechanism of hematoxylin was studied in phosphate buffers and 0.1 M KCl by cyclic voltammetry and UV-Vis spectroscopy under deaerated conditions. The redox potential of hematoxylin in buffered solution strongly depends on pH. A two electron oxidation is preceded by deprotonation. The homogeneous rate of deprotonation process of hematoxylin in 0.1 M phosphate buffer is kd = (2.5 ± 0.1) × 104 s -1. The cyclic voltammetry under unbuffered conditions shows the distribution of various dissociation forms of hematoxylin. The dissociation constants pK1 = 4.7 ± 0.2 and pK2 = 9.6 ± 0.1 were determined using UV-Vis spectroscopy. The final oxidation product was identified by gas chromatography with mass spectrometry detection as hemathein. The distribution of oxidation products differs under buffered and unbuffered conditions. The dye degradation in natural unbuffered environment yields hemathein and hydroxyhematoxylin, which is absent in buffered solution.
Sokolova, Romana,Degano, Ilaria,Hromadova, Magdalena,Bulickova, Jana,Gal, Miroslav,Valasek, Michal
p. 1097 - 1114
(2010)
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More Articles about upstream products of 475-25-2

17647-60-8
hematoxylin

Chemical Properties

Appearance/Colour :dark brown crystalline powder
Melting Point: 180 °C
Boiling Point: 753.1 °C at 760 mmHg
Flash Point:289 °C
Density: 1.77g/cm³
Solubility:Methanol (Slightly)
Storage Temp.:2-8°C
Vapor Pressure: 1.32E-17mmHg at 25°C
Refractive Index: 1.765
PKA: 8.02±0.40(Predicted)
Merck: 13,4651
BRN: 93039
PSA: 107.22000
LogP: 1.32960

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