Photolabeling of dextransucrase from Streptococcus sanguis with p-azidophenyl α-D-glucopyranoside
Dextransucrase from Streptococeus sanguis ATCC 10558 was photolabeled using p-azidophenyl α-D-glucopyranoside with an apparent rate constant of inactivation of 1.40 min-1. The dissociation constant for this compound, which acts as an acceptor m
Post-photoaffinity labeling modification using aldehyde chemistry to produce a fluorescent lectin toward saccharide-biosensors
A new method to construct a fluorescent saccharide biosensor based on a lectin protein is successfully proposed using post-photoaffinity labeling modification coupled to aldehyde chemistry.
Nagase,Shinkai,Hamachi
p. 229 - 230
(2007/10/03)
Syntheses and properties of photoactivatable sugar derivatives designed to probe the sugar-binding site of melibiose permease
Three new photoreactive sugar analogues bearing an azido, a diazonium salt or a diazirine group as the photophore as well as a tritium atom were developed. Two of these new photoactivatable compounds gave excellent preliminary results, with a high affinity for the melibiose permease of Escherichia coli. (C) 2000 Elsevier Science Ltd. All rights reserved.
Ambroise, Yves,Mioskowski, Charles,Leblanc, Gerard,Rousseau, Bernard
p. 1125 - 1127
(2007/10/03)
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