- AGENTS FOR CLEARING BIOMOLECULES FROM CIRCULATION
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Described is a method, and a combination of agents for used therein, by which an agent administered to a subject can be rapidly cleared from circulation. This is achieved by providing an Administration Agent (e.g. a probe for pretargeting) with a reactive group and providing a Clearing Agent with another reactive group, said reactive groups forming a bio-orthogonally reactive pair. Preferably, the reactive pair comprises a cyclooctene or cyclooctyn as one reactant, and a diene as the other reactant. The method and combination can be used for the removal of any bindable molecule from circulation, such as an excess of a pre-targeting probe in the course of a pre-targeting method, a targeting or imaging agent delivered, or the removal of any biomolecule already present in circulation.
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- Glycoviruses: Chemical glycosylation retargets adenoviral gene transfer
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A sugar-specific transfection mechanism is introduced by the glycosylation of adenoviruses (AVs, see picture), and manipulation of the glycosylation pattern allows the selective transfection of human macrophages in favor of the usual target. This dramatic retargeting holds promise for the fine-tuning of adenoviruses for applications such as gene therapy.
- Pearce, Oliver M. T.,Fisher, Kerry D.,Humphries, Julia,Seymour, Leonard W.,Smith, Alberto,Davis, Benjamin G.
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p. 1057 - 1061
(2007/10/03)
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