- Grid coatings for capture of proteins and other compounds
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Grids comprising a coating modified with one or more capture agents and a deactivating agent are disclosed. Methods of using such grids in connection with suitable microscopy techniques, such as for determining the structure of target compounds including proteins, are also disclosed.
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Page/Page column 19-21
(2021/04/28)
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- SWCNT-porphyrin nano-hybrids selectively activated by ultrasound: an interesting model for sonodynamic applications
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Sonodynamic therapy (SDT) is an innovative anticancer approach, based on the excitation of a given molecule (usually a porphyrin) by inertial acoustic cavitation that leads to cell deathviathe production of reactive oxygen species (ROS). This study aims t
- Arpicco, Silvia,Barge, Alessandro,Bosca, Federica,Canaparo, Roberto,Corazzari, Ingrid,Cravotto, Giancarlo,Dosio, Franco,Durando, Gianni,Foglietta, Federica,Pastero, Linda,Serpe, Loredana,Tagliapietra, Silvia,Turci, Francesco,Zonari, Daniele
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p. 21736 - 21744
(2020/07/03)
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- Optimized reaction pair of the Cyshis tag and Ni(II)-Nta probe for highly selective chemical labeling of membrane proteins
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Chemical labeling of proteins with synthetic molecular probes offers the possibility to probe the functions of proteins of interest in living cells. However, the methods for covalently labeling targeted proteins using complementary peptide tag-probe pairs are still limited, irrespective of the versatility of such pairs in biological research. Herein, we report the new CysHis tag-Ni(II) probe pair for the specific covalent labeling of proteins. A broad-range evaluation of the reactivity profiles of the probe and the CysHis peptide tag afforded a tag-probe pair with an optimized and high labeling selectivity and reactivity. In particular, the labeling specificity of this pair was notably improved compared to the previously reported one. This pair was successfully utilized for the fluorescence imaging of membrane proteins on the surfaces of living cells, demonstrating its potential utility in biological research.
- Zenmyo, Naoki,Tokumaru, Hiroki,Uchinomiya, Shohei,Fuchida, Hirokazu,Tabata, Shigekazu,Hamachi, Itaru,Shigemoto, Ryuichi,Ojida, Akio
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p. 995 - 1000
(2019/07/18)
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- TREATING BLADDER TUMOR CELLS USING FIBRONECTIN ATTACHMENT PROTEIN AS A TARGET
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Composition and methods are disclosed for utilizing microaggregation of FAP-containing complexes to promote their fast internalization. This approach allows the uptake of cytotoxic cargo coupled to either FAP-Antibodies or FAP-liposome complexes by tumor
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Paragraph 0081
(2017/07/28)
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- Development of nonfouling polypeptides with uniform alternating charges by polycondensation of the covalently bonded dimer of glutamic acid and lysine
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In this work, nonfouling polypeptides with homogenous alternating charges were synthesized by polycondensation of the covalently bonded dimer of glutamic acid (E) and lysine (K) (EK dimer) with benzyloxycarbonyl (Z)-protected side chains. This facile method successfully solved the uniformity problem of nonfouling peptides caused by the copolymerization of two different monomers and enabled the incorporation of various terminal functional groups for future applications. The molecular weights (MWs) of the nonfouling peptides can be easily controlled by the ratio of the terminal group, lipoic acid, to the EK dimer. The nonfouling peptides can form self-assembling monolayers (SAMs) on a gold surface through two terminal thiol groups, which were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ellipsometry (ELL). The resistance to nonspecific protein adsorption, cell attachment and bacterial adhesion of these nonfouling peptide SAMs and the in vitro cytotoxicity and haemolytic activity of these peptides were also evaluated. The results show that the lowest relative protein adsorptions of antibody (anti-IgG) and fibrinogen (Fg) on the SAMs are 5.1 ± 1.6% and 7.3 ± 1.8%, respectively, determined by enzyme-linked immunosorbent assay (ELISA), where the protein adsorption on a tissue culture polystyrene (TCPS) surface was set to 100%. Almost no obvious cell attachment and bacterial adhesion were observed, and no cytotoxicity and no haemolytic activity in vitro were detected. With the advantages of biocompatibility, biodegradability and the abundance of moieties for ligand immobilization, these nonfouling peptides developed by the facile method can be used in a wide range of biomedical applications. The Royal Society of Chemistry 2014.
- Yang, Qinghua,Wang, Longgang,Lin, Weifeng,Ma, Guanglong,Yuan, Jiang,Chen, Shengfu
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p. 577 - 584
(2014/01/17)
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- Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles
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Ni2+:NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is co
- Hyun, Seok-Hee,Kim, Hee-Kwon,Kim, Jong-Mok,Thompson, David H.
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supporting information; experimental part
p. 17053 - 17055
(2011/03/01)
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- Synthesis of nickel-chelating fluorinated lipids for protein monolayer crystallizations
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Nickel-chelating lipids have been synthesized for use as functionalized templates for 2-D crystallization of membrane proteins. These monolayer-forming lipids have been designed with three distinct components: (i) a branched hydrocarbon tail to confer flu
- Hussein, Waleed M.,Ross, Benjamin P.,Landsberg, Michael J.,Lévy, Daniel,Hankamer, Ben,McGeary, Ross P.
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supporting information; experimental part
p. 1473 - 1479
(2009/07/11)
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- 2,2-Dimethyl-2-(o-nitrophenyl)acetyl (DMNA) as an assisted cleavage protecting group for amines
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2,2-Dimethyl-2-(o-nitrophenyl)acetyl group (DMNA) was explored as an assisted cleavage protecting group for amines and a one-step deprotection condition was developed for its efficient removal using hydrogenation in the presence of Pd-C or PtO2 catalyst and 10% HOAc in MeOH. DMNA was found to be especially useful for the synthesis of gem-diamino compounds using Hofmann rearrangement.
- Jiang, Yongying,Zhao, Jun,Hu, Longqin
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p. 4589 - 4592
(2007/10/03)
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- Selective nitrolytic deprotection of N-BOC-amines and N-BOC-amino acids derivatives
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The extension of the deprotection procedure using HNO3 in CH2Cl2 to a number of appropriately selected N-BOC-masked amines and derivatives of natural amino acids was investigated. The method was found to work effectively with almost all tested substrates, with the exception of activated aromatic amines and heterocycles which underwent unavoidable faster oxidation. Alanine, phenylalanine, serine and lysine derivatives were efficiently deprotected, as well as dipeptide Ala-Phe, preserving the configuration of the substrates and without affecting copresent Z and ester functions, with a remarkable selectivity towards acid sensitive t-butyl esters. The obtained amino acids esters, isolated and characterized in the form of nitrates salts, proved to be suitable intermediates to be used in peptide synthesis.
- Strazzolini, Paolo,Melloni, Tiziana,Giumanini, Angelo G
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p. 9033 - 9043
(2007/10/03)
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- Stabilized analogs of thymopentin. 2. 1,2- and 3,4-ketomethylene pseudopeptides
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In this second paper in a series of three studies of stable analogs of thymopentin (Arg1-Lys2-Asp3-Val4-Tyr5), the synthesis of analogs stablized at peptide bonds 1,2 and 3,4 via insertion of ketomethylene units is described. A tris(carbobenzyloxy)arginyl(k)norleucine pseudopeptide was synthesized and coupled to Asp-Val-Phe-resin units followed by HF cleavage to prepare Arg(k)Nle-Asp-Val-Phe analogs. Preparation of N-BOC Asp(k)Val and N- BOC Asp(k)Ala units followed by coupling to Phe- or Tyr-resin units provided resin-bound pseudotripeptide substrates for attachment of various arginyl dipeptides. Cleavage from the resin afforded 3,4-ketomethylene-stabilized pseudopeptide analogs of thymopentin. The Arg-Lys-Asp(k)Val-Phe and ArgLys- Asp(k)Val-Tyr analogs were more strongly bound to CEM cells than thymopentin itself. There was significant enhancement of stability in serum for the analogs, especially those containing Arg(k)Nle or Arg-NMeLys moieties at the 1,2-peptide bond.
- DeGraw, Joseph I.,Almquist, Ronald G.,Hiebert, Charles K.,Judd, Amrit K.,Dousman, Linda,Smith, R. Lane,Waud, William R.,Uchida, Itsuo
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p. 2398 - 2406
(2007/10/03)
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- Angiotensin-converting enzyme inhibitors. 9. Novel [[N-(1-carboxy-3-phenylpropyl)amino]acyl]glycine derivatives with diuretic activity
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A series of molecules 1 having sulfonamide diuretic moieties covalently linked to non-sulfhydryl angiotensin-converting enzyme inhibitors (ACEI) were prepared and tested for both activities. IC50 values for ACEI as low as 7 nM were observed. Di
- Barton,Piwinski,Skiles,Regan,Menard,Desai,Golec,Reilly,Goetzen,Ueng,Warus,Schwab,Samuels,Neiss,Suh
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p. 1600 - 1606
(2007/10/02)
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- Compounds for treating hypertension
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Compounds of the formula STR1 and their pharmaceutically acceptable salts, wherein the substituents are as defined herein, having antihypertensive activity.
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- COMPOUNDS FOR TREATING HYPERTENSION
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Compounds of the formula STR1 and their pharmaceutically acceptable salts, wherein the substituents are as defined herein, having antihypertensive activity.
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- Synthesis and Biological Activity of some Fused &β-Lactam Peptidoglycan Analogues
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The derivatives (12), (14), (15), (19), and (22), in which a mono- or di-peptide unit is linked to 6-aminopenicillanic acid, have been prepared according to normal synthetic peptide procedures.The structures of the side chains were chosen by analogy with the D-isoglutamyl-L-lysyl sequence present in the cell-wall peptidoglycan of Staphylococcus species.The antibacterial activities of the derivatives were measured and the results are discussed.
- Bentley, Peter H.,Stachulski, Andrew V.
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p. 1187 - 1192
(2007/10/02)
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