- Isolation of nucleic acids
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A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
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- Amino-hydroxy-alkyl sulfonic acid zwitterions
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Novel amino-hydroxy-alkylene sulfonic acid zwitterions are described which have the general formulas STR1 wherein A and B are each independently a hydrogen atom, an aliphatic, cycloaliphatic or hydroxyaliphatic group, N is one or two, and R=n is a cyclic radical comprising one or two ring nitrogen atoms, zero or one ring oxygen atoms and the remainder of the ring being carbon atoms, and water soluble salts of said acids. These organo-sulfonic acid compounds are useful as hydrogen ion buffers in biological research and particularly for tissue culture.
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