- Development of a selective activity-based probe for glycosylated LIPA
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Loss of LIPA activity leads to diseases such as Wolman's Disease and Cholesterol Ester Storage Disease. While it is possible to measure defects in LIPA protein levels, it is difficult to directly measure LIPA activity in cells. In order to measure LIPA activity directly we developed a LIPA specific activity based probe. LIPA is heavily glycosylated although it is unclear how glycosylation affects LIPA activity or function. Our probe is specific for a glycosylated form of LIPA in cells, although it labels purified LIPA regardless of glycosylation.
- Schwaid, Adam G.,Ruangsiriluk, Wanida,Reyes, Allan R.,Cabral, Shawn,Rajamohan, Francis,Tu, Meihua,Ward, Jessica,Carpino, Philip A.
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supporting information
p. 1993 - 1996
(2016/04/05)
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- FUSED THIOPHENE DERIVATIVES AS KINASE INHIBITORS
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A series of 5,6-dihydro-1-benzothiophen-7(4H)-one derivatives, and analogues thereof, which are substituted in the 2-position by an optionally substituted morpholin-4-yl moiety, being selective inhibitors of PI3 kinase enzymes, are accordingly of benefit in medicine, for example in the treatment of inflammatory, autoimmune, cardiovascular, neurodegenerative, metabolic, oncological, nociceptive or ophthalmic conditions.
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Page/Page column 59
(2008/06/13)
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