Development of monoclonal ELISAs for azinphos-methyl. 1. Hapten synthesis and antibody production
The development of monoclonal antibody-based enzyme-linked immunosorbent assays for azinphosmethyl is described. A panel of haptens was synthesized for immunoconjugate preparation, and a series of haptens for heterologous, coating or tracer, conjugates was also prepared. Hapten synthesis was based on a strategy in which only a fragment of the whole target molecule was present (fragmentary haptens). From immunized mice, a set of monoclonal antibodies was obtained and ELISA sensitivities were assayed in different formats. Affinities estimated as I50 values in the low nanomolar range for azinphos-methyl and phosmet were observed for several monoclonal antibodies in the conjugate-coated format and in the antibody-coated format under nonoptimized assay conditions.
Mercader, Josep V.,Montoya, Angel
p. 1276 - 1284
(2007/10/03)
Quinazoline carboxylic acids. An easy route to (4-Oxo-3,4-dihydroquinazolin-3-yl)-alkanoic acids, (4-Oxo-3,4-dihydro-1,2,3-benzotriazin-3-yl)-alkanoic acids and their esters
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Susse,Johne
p. 892 - 899
(2007/10/02)
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