Removal of extraneous materials (impurities) from a substance or mixture by one or more separation techniques. A pure substance is one in which no impurity can be detected by any experimental procedure. Though absolute purity is impossible to attain, a number of standard procedures exist for approaching it to the extent of 1 ppm of impurity or less. The following fractionation techniques are widely used: crystallization, precipitation, distillation, adsorption (various types of chromatography), extraction, electrophoresis, and thermal diffusion.See Purity, Chemical.