Yeast Artificial Chromosome

Name:
Yeast Artificial Chromosome
Detailed information:
(YAC). Constructed from yeast DNA, it is a vector used to clone large DNA fragments; a method for cloning very large fragments of DNA. Genomic DNA in fragments of 200-500 kb are linked to sequences which allow them to propagate in yeast as a mini-chromosome (including telomeres, a centromere, and an ARS—an autonomous replication sequence). This technique is used to clone large genes and intergenic regions, and for chromosome walking.See Cloning Vector; Cosmid.