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Ethidium bromide

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Ethidium bromide

EINECS 214-984-6
CAS No. 1239-45-8 Density g/cm3
PSA 55.92000 LogP 2.29820
Solubility 40 g/L (25 °C) in water Melting Point 260-262 °C (dec.)(lit.)
Formula C21H20BrN3 Boiling Point N/A
Molecular Weight 394.314 Flash Point >100°C
Transport Information UN 2811 6.1/PG 1 Appearance Purple/maroon crystalline powder
Safety 36/37-45-36/37/39-28A-26-22-28 Risk Codes 23-68-36/37/38-26-21/22-22
Molecular Structure Molecular Structure of 1239-45-8 (Ethidium bromide) Hazard Symbols ToxicT, VeryT+
Synonyms

3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI);Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI);2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide;2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide;2,7-Diamino-9-phenylphenanthridine ethobromide;Dromilac;Homidium bromide;

Article Data 9

Ethidium bromide Chemical Properties

Molecular Structure:

Molecular Formula: C21H20BrN3
Molecular Weight: 394.3076
IUPAC Name: 5-Ethyl-6-phenylphenanthridin-5-ium-3,8-diamine bromide
Synonyms of Ethidium bromide (CAS NO.1239-45-8): 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide  2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide ; 2,7-Diamino-9-phenylphenanthridine ethobromide ; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide ; AI3-62997 ; Bromure d'homidium ; Bromure d'homidium [INN-French] ; Bromuro de homidio ; Bromuro de homidio [INN-Spanish] ; CCRIS 1017 ; Dromilac ; EINECS 214-984-6 ; Ethydium bromide ; HSDB 7297 ; Homidii bromidum ; Homidii bromidum [INN-Latin] ; Homidium bromide ; NSC 268986 ; RD 1572 ; UNII-059NUO2Z1L ; 3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide ; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide  ; Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide
CAS NO: 1239-45-8
Classification Code: Drug / Therapeutic Agent ; Mutation data ; Tumor data
Melting point: 260-262 °C 
H bond acceptors: 3
H bond donors: 4
Freely Rotating Bonds: 4
Polar Surface Area: 10.36 Å2 
Water solubility: 40 g/L (25 ºC)

Ethidium bromide Toxicity Data With Reference

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
mouse LD50 subcutaneous 110mg/kg (110mg/kg)   Annals of Tropical Medicine and Parasitology. Vol. 46, Pg. 285, 1952.
mouse LDLo intraperitoneal 20mg/kg (20mg/kg)   Cancer Research. Vol. 34, Pg. 2699, 1974.

Ethidium bromide Consensus Reports

EPA Genetic Toxicology Program.

Ethidium bromide Safety Profile

Hazard Codes of Ethidium bromide (CAS NO.1239-45-8): ToxicT,VeryT+
Risk Statements: 23-68-36/37/38-26-21/22 
R23: Toxic by inhalation. 
R68: Possible risk of irreversible effects. 
R36/37/38: Irritating to eyes, respiratory system and skin. 
R26: Very toxic by inhalation. 
R21/22: Harmful in contact with skin and if swallowed.
Safety Statements: 45-36/37/39-26-22-28 
S45: In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) 
S36/37/39: Wear suitable protective clothing, gloves and eye/face protection. 
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 
S22: Do not breathe dust. 
S28: After contact with skin, wash immediately with plenty of soap-suds.
RIDADR: UN 2811 6.1/PG 1
WGK Germany :3
RTECS: SF7950000
F: 8-9
HazardClass: 6.1
PackingGroup: I
HS Code: 29339990
Poison by intraperitoneal and subcutaneous routes. Human mutation data reported. When heated to decomposition it emits very toxic fumes of NOx and Br. See also BROMIDES.

Ethidium bromide Specification

 Ethidium bromide (CAS NO.1239-45-8) is a purple/maroon crystalline powder and it is stable. It is partially soluble in cold water. It should keep in a cool, well-ventilated place.

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