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Acknowledgment
This work was supported by Grants-in-Aid for Scientific Re-
search from the Japan Society for the Promotion of Science (JSPS).
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Supplementary data
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12. MALDI-TOF/MS analyses of ONs. The following spectra were obtained by MALDI-
TOF/MS (negative mode). ON 14: calculated mass, 6282.8; observed mass,
6282.9. ON 15: calculated mass, 6591.9; observed mass, 6595.3. ON 17:
calculated mass, 7130.1; observed mass, 7128.1.
Supplementary data associated with this article can be found, in
13. Thermal denaturation study. Each solution containing each siRNA (3.0 lM) in a
References and notes
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each mixture were monitored at 260 nm with a spectrophotometer.
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l
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7.0) and 7.5 mM MgCl2 (total 100
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urea (15 L). The mixtures were analyzed by electrophoresis on 20%
lL) at 37 °C. At appropriate periods, aliquots
l
l
polyacrylamide gel containing 7.0 M urea. The labeled ON in the gel was
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