450
PILYUGIN et al.
IX–XX (general procedure). Finely powdered calcium
cyanamide containing 27.5% of the main substance, 24 g,
was dispersed in 900 ml of water, and the
corresponding substituted benzoyl chloride or
benzenesulfonyl chloride (solid benzoyl chlorides were
added as solutions in anhydrous acetone), 0.33 mol,
was added under vigorous stirring at such a rate that
the temperature did not exceed 40–45°C. The mixture
was stirred for 1 h at 40–45°C, cooled to room
temperature, and filtered from inorganic salts present
in the initial calcium cyanamide. The filtrate was
adjusted to pH 3 by adding concentrated hydrochloric
acid, and the precipitate of RC(O)NHCN·HCl was
filtered off and dried in air in a hood. Depending on
the initial acyl chloride, the yield of the complex RC
(O)NHCN·HCl varied from 7.6 (5-chloro-2-
hydroxybenzoyl chloride) to 95% (benzenesulfonyl
chloride).
Potato glucose agar was used as substrate. Compounds
IX–XX were applied to the nutrient medium before
solidification (at 45–50°C), and it was then poured
onto Petri dishes in 15-cm3 portions. Each Petri dish
was inoculated with Fusarium culmorum and
Helminthosporium sativum mycelia simultaneously at
three points. In blank experiment, the fungal cultures
were inoculated into the same nutrient medium
containing no fungicides. The dishes were incubated
for 72 h at 25–26°C, and the average diameter of the
colonies was measured.
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231°C; XV, 162°C; XVI, 89°C; XVII, 153°C; XVIII,
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RUSSIAN JOURNAL OF GENERAL CHEMISTRY Vol. 78 No. 3 2008