Organic and Biomolecular Chemistry p. 1007 - 1013 (2012)
Update date:2022-08-04
Topics:
Kanamori, Takashi
Masaki, Yoshiaki
Mizuta, Masahiro
Tsunoda, Hirosuke
Ohkubo, Akihiro
Sekine, Mitsuo
Seio, Kohji
We have previously reported DNA triplexes containing the unnatural base triad G-PPI·C3, in which PPI is an indole-fused cytosine derivative incorporated into DNA duplexes and C3 is an abasic site in triplex-forming oligonucleotides (TFOs) introduced by a propylene linker. In this study, we developed a new unnatural base triad A-ψ·CR1 where ψ and CR1 are base moieties 2′-deoxypseudouridine and 5-substituted deoxycytidine, respectively. We examined several electron-withdrawing substituents for R1 and found that 5-bromocytosine (C Br) could selectively recognize ψ. In addition, we developed a new PPI derivative, PPIMe, having a methyl group on the indole ring in order to achieve selective triplex formation between DNA duplexes incorporating various Watson-Crick base pairs, such as T-A, C-G, A-ψ, and G-PPIMe, and TFOs containing T, C, CBr, and C3. We studied the selective triplex formation between these duplexes and TFOs using UV-melting and gel mobility shift assays.
View MoreJiaozuo Zhongwen Trading Coporation Limited
Contact:+86 0391-3553810
Address:East Renmin Road
Contact:86-512-69362780,69362785
Address:No.69 Weixin Road,Weiting Town,Suzhou Industrial Park
Lonzeal Pharmaceuticals Co., Ltd.
website:http://www.lonzeal.com
Contact:+86-13381011962
Address:RM 801, Yue MOMA, No. 26 Anningzhuang Rd. Haidian District, Beijing, China
Lianyungang Yunbo Chemical Co.,Ltd.
Contact:518-81066110
Address:B907,Dongsheng"Mingdu Square,21-2 East Chaoyang Road,Xinpu,(sale department)
Zouping Mingxing Chemical Co.,Ltd.
website:http://www.zoutong.com.cn
Contact:86-543-2240068 2240067
Address:428 Daixi Third Road Zouping County Shandong Province China
Doi:10.1016/S0020-1693(00)85192-0
(1991)Doi:10.1021/acs.oprd.1c00033
(2021)Doi:10.1016/j.bmcl.2012.05.040
(2012)Doi:10.1016/j.molstruc.2012.04.023
(2012)Doi:10.1007/BF00590576
(1969)Doi:10.1021/ja00030a056
(1992)