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12. Analytical data and physicochemical properties of the
compound 2. 1H NMR (CDCl3): d 0.93–1.07 (m, 12H),
1.62–1.84 (m, 5H), 2.27 (s, 3H), 2.53 (m, 1H), 3.08 (t, 1H),
3.42 (d, 2H), 3.72–3.85 (m, 3H), 4.28–4.63 (m, 6H), 6.86–
7.12 (m, 4H), 7.20–7.45 (m, 5H); HPLC: KI=7.31; MS:
M+H+=591.7. [a]2D0=À39.7 (c=1, MeOH). Anal. calcd
for C29H42N4O9: C, 58.97, H, 7.17, N, 9.49, O, 24.38.
Found: C, 58.23, H, 7.04, N, 9.34. Compound 3. 1H
NMR (CDCl3): d 0.89–1.11 (m, 12H), 1.62–1.84 (m, 5H),
2.07 (t, 2H), 2.53 (m, 1H), 2.84 (t, 3H), 3.08 (t, 1H), 3.29–
3.41 (m, 6H), 3.68–3.80 (m, 3H), 4.18–4.42 (m, 6H), 6.71–
7.05 (m, 4H); HPLC: KI=8.09; MS: M+H+=566.5.
[a] 2D0=À33.4 (c=1, MeOH). Anal. calcd for C27H43N5O8:
C, 57.33, H, 7.66, N, 12.38, O, 22.63. Found: C, 56.85, H,
7.26, N, 12.03.
13. Purification of the proteasomes and enzyme assays are
described in ‘supporting information’ of the ref 7.
14. Enzyme assays in live cells are described in ‘supporting
information’ of ref 7.
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