506
J. Kroutil, J. Karban / Carbohydrate Research 340 (2005) 503–506
Organic layers were washed with 5% HCl, 10% NaH-
CO3, and water. After drying over anhyd Na2SO4 and
evaporation of the solvent, the residue was chromato-
graphed on silica gel (150 g) with 3:1–3:2–1:1 hexane–
EtOAc to give a colorless oil. The oil crystallized in
the refrigerator (À30 ꢁC) after a while. Analytical sam-
ples were recrystallized (EtOAc–Et2O–PE) to afford
pure nosylepimine 9. Yield 2.44 g (58%); mp 122–
124 ꢁC; [a]D +1.5 (c 0.33 CHCl3). Anal. Calcd for
C19H18N2O7S: C, 54.54; H, 4.34; N, 6.70; S, 7.66.
Found: C, 54.38; H, 4.41; N, 6.59; S, 7.71.
Acknowledgements
The authors are grateful for financial support of the
Grant Agency of the Czech Republic (grant no. 203/
02/D047) and the Ministry of Education, Youth and
Sports of the Czech Republic (MSM 113100001).
References
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1,6-Anhydro-4-azido-2-O-benzyl-4-deoxy-3-O-tosyl-b-
D-glucopyranose (7)1 (4.34 g, 10.05 mmol), NaBH4
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