Bioorganic and Medicinal Chemistry p. 1369 - 1375 (2016)
Update date:2022-08-17
Topics:
Miura, Kazuki
Hakamata, Wataru
Tanaka, Ayako
Hirano, Takako
Nishio, Toshiyuki
Post-translational modifications (PTMs) of proteins play important roles in the physiology of eukaryotes. In the PTMs, non-reversible glycosylations are classified as N-glycosylations and O-glycosylations, and are catalyzed by various glycosidases and glycosyltransferases. However, β-glycosidases are not known to play a role in N- and O-glycan processing, although both glycans provide partial structures as substrates for β-galactosidase and β-N-acetylglucosaminidase in the Golgi apparatus of human cells. We explored human Golgi β-galactosidase using fluorescent substrates based on a quinone methide cleavage (QMC) substrate design platform that was previously developed to image exo-type glycosidases in living cells. As a result, we discovered a novel Golgi β-galactosidase in human cells. It is possible to predict a novel and important function in glycan processing of this β-galactosidase, because various β-galactosyl linkages in N- and O-glycans exist in Golgi apparatus. In addition, these results show that the QMC platform is excellent for imaging exo-type glycosidases.
View MoreMTT Pharma & Bio-technology Co.,Ltd
Contact:+86-21-58407925
Address:Room2019, Building C, Tomson Center, No.158, Zhang Yang Road, Shanghai, China
Hebei DaPeng Pharm & Chem Co., Ltd.
Contact:86-317-7298678,0576-88861898 88861908
Address:West Songmen Village, East Songmen Town, Wuqiao, Cangzhou, Hebei ,China
Shanghai Pengkai Chemical Co.,Ltd
Contact:+86-21-60526671
Address:No.4226 Duzhuang Rd Minhang District Shanghai China
Xian Changyue Biological Technology Co., Ltd.
website:https://www.xachangyue.com/
Contact:+86-029-62886900
Address:Keji Road NO.70
Shanghai Run-Biotech Co., Ltd.
website:http://www.run-biotech.com
Contact:+86-21-31576854/57171705 / 57171706
Address:second floor, building 3, No.999, jiangyue Road, minghang District, Shanghai, China
Doi:10.1016/j.poly.2013.06.002
(2013)Doi:10.1039/c9cc06831g
(2019)Doi:10.1002/anie.201409145
(2015)Doi:10.1016/j.tetlet.2021.152868
(2021)Doi:10.1016/j.tetasy.2003.11.004
(2004)Doi:10.1246/bcsj.31.550
(1958)