LIU ET AL.
7
6. Shen J, Okamoto Y. Efficient separation of enantiomers using
stereoregular chiral polymers. Chem Rev. 2016;116(3):1094‐1138.
the amino groups in the amino acid structure, while pay-
ing attention to the ionic interaction between the
hydroxyls groups and amino groups of APTES.
7. Armstrong DW, Tang YB, Ward T, Nichols M. Derivatized
cyclodextrins immobilized on fused‐silica capillaries for enantio-
meric separations via capillary electrophoresis, gas
chromatography, or supercritical fluid chromatography. Anal
Chem. 1993;65(8):1114‐1117.
4 | CONCLUSION
The present work presents a separation procedure in
which heparin are added to the BGE as a pseudo station-
ary phase. Although the use of heparin as a pseudo sta-
tionary phase has been previously described by other
colleagues, in this work the separation procedure is
improved thanks to the presence of APTES attached to
the inner phase of the capillary. In general, stronger
interactions, as evidenced by higher resolution, were
observed between heparin and tryptophan. The method
described in this study are very promising in terms of
resolution and reproducibility. Further work including
expanding the number and types of solutes to assist in
elucidating solute structural features required for success-
ful enantioselective interactions, examining the role of
the molecular weight, and the degree of sulfation for
heparin must be done.
8. Declerck S, Vander Heyden Y, Mangeling D. Enantioseparations
of pharmaceuticals with capillary electrochromatography: a
review. J Pharm Biomed Anal. 2016;130:81‐99.
9. Kapnissi‐Christodoulou
C,
Nicolaou
A,
Stavrou
I.
Enantioseparations in open‐tubular capillary electrochroma-
tography: recent advances and applications. J Chromatogr A.
2016;1467:145‐154.
10. Liu Y, Jann M, Vandenbergc C, Eap C, Shamsi S. Development
of an enantioselective assay for simultaneous separation of
venlafaxine and O‐desmethylvenlafaxine by micellar electroki-
netic chromatography‐tandem mass spectrometry: application
to the analysis of drug‐drug interaction. J Chromatogr A.
2015;1420:119‐128.
11. Baciu T, Botello I, Borrull F, Calull M, Aguilar C. Capillary elec-
trophoresis and related techniques in the determination of drugs
of abuse and their metabolites. Trends Anal Chem. 2015;74:
89‐108.
12. Tait RJ, Thompson DO, Stella VJ, Stobaugh JF. Sulfobutyl ether
beta‐cyclodextrin as a chiral discriminator for use with capillary
electrophoresis. Anal Chem. 1994;66(22):4013‐4018.
ACKNOWLEDGMENTS
This work was supported by Consejo Nacional de
Investigaciones Científicas y Técnicas (CONICET PIP
11220150100768CO) and Universidad Nacional de San
Luis (PROICO 2‐2816)
13. Armstrong DW, Nair UB. Capillary electrophoretic enantiosepa-
rations using macrocyclic antibiotics as chiral selectors.
Electrophoresis. 1997;18(12‐13):2331‐2342.
14. Kuhn R, Stocklin F, Erni F. Chiral separations by host‐guest
complexation with cyclodextrin and crown ether in capillary
zone electrophoresis. Chromatographia. 1992;33(1‐2):32‐36.
ORCID
15. Ishihama Y, Terabe S. Enantiomeric separation by micellar elec-
trokinetic chromatography using Saponins. J Liq Chromatogr.
1994;16:933‐944.
16. Mazzeo JR, Grover ER, Swartz ME, Petersen JS. Novel chiral
surfactant for the separation of enantiomers by micellar electro-
REFERENCES
kinetic capillary chromatography.
1994;680(1):125‐135.
J
Chromatogr A.
1. Amina N, Blanchin M, Aké M, Review FH. Capillary electro-
phoresis methods for the analysis of antimalarials. Part I.
J Chromatogr A. 2012;1264:1‐12.
17. Li S, Lloyd DK. Direct chiral separations by capillary electropho-
resis using capillaries packed with an alpha1‐acid glycoprotein
chiral stationary phase. Anal Chem. 1993;65(24):3684‐3690.
2. Gilar M, Tesarova E, Patzelova V, Deyl Z. Chiral separation of
pharmacologically important substances by HPLC. Chem Listy.
1994;88:514‐526.
18. Belder D, Schomburg G. Chiral separations of basic and acidic
compounds in modified capillaries using cyclodextrin‐modified
capillary zone electrophoresis. J Chromatogr A. 1994;666(1‐2):
351‐365.
3. Allenmark SG, Andersson S. Proteins and peptides as chiral
selectors in liquid chromatography.
J
Chromatogr A.
1994;666(1‐2):167‐179.
19. Li S, Lloyd DK. Packed‐capillary electrochromatographic sepa-
ration of the enantiomers of neutral and anionic compounds
using β‐cyclodextrin as a chiral selector: effect of operating
parameters and comparison with free‐solution capillary electro-
phoresis. J Chromatogr A. 1994;666:321‐335.
4. Haginaka J, Murashima T, Seyama C. Separation of enantio-
mers on a lysozyme‐bonded silica column. J Chromatogr A.
1994;666(1‐2):203‐210.
5. Roussel C, Favrou A. Cationic β‐cyclodextrin: a new versatile
chiral additive for separation of drug enantiomers by high‐
20. Valtcheva L, Mohammad J, Pettersson G, Hjertén S. Chiral
separation of β‐blockers by high‐performance capillary electro-
performance liquid chromatography.
J
Chromatogr A.
phoresis
based
on
non‐immobilized
cellulase
as
1995;704(1):67‐74.
enantioselective protein. J Chromatogr. 1993;638(2):263‐267.