Month 2018
Imidazo[1,2-a]pyridine Derivatives as Potential Quorum Sensing Inhibitors
1
1
1
17.58 (C5); 117.98 (C8); 119.03 (C3); 123.74 (C7);
26.07; 128.44 (Ca); 128.72 (Cb); 132.55; 133.26;
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[
35.89 (Cc); 142.52; 151.44; 162.75 (C═O); 168.70
+
(C═O). RT: 2.21 min. MS: m/z: 353.2 [M + H] ; 354.2
+
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[
M + H + 1] . Anal. Calcd for C H N O : C 61.36, H
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[
Biological evaluation. Quorum sensing inhibition in liquid
1
medium. Two milliliters of C. violaceum CV026 strain
NCTC 13278) in LB MOPS (3-(N-morpholine)
[
(
7
propanesulfonate) broth (10 CFU/mL) added with
C8-AHL (0.25 mg/mL; Sigma-Aldrich) were transferred
into a conic tube of 50 mL and incubated with its
respective treatments during 18 h at 30°C. Compounds
[
Hodgkinson, J. T.; O’Connell, K. M.; Bartolucci, N.; Della-Valle, M.;
Welch, M.; Spring, D. R. Org Biomol Chem 2012, 10, 8452.
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2
2
015, 51, 1555.
5a to 5d and 9a to 9d were dissolved in DMSO and
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015, 47, 887.
added at final concentration of 100 μM. After incubation,
bacterial culture was centrifuged (10 min; 10,000 rpm),
and LB broth was removed, and cellular package was
resuspended in 1 mL of DMSO and recentrifuged
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violacein pigment were transferred to microwell plate by
triplicate, and its OD was recorded on Thermo Scientific
spectrophotometer at 595 nm. Violacein OD was used as
indicative of communication process and its effectiveness.
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CONFLICT OF INTEREST
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[
The authors confirm that the article content has no
conflict of interest.
[
2
[
30] Rival, Y.; Grassy, G.; Taudou, A.; Ecalle, R. Eur J Med Chem
Acknowledgments. This work was supported financially by
CONACyT with the scholarship numbers 254499, CB-222293,
and 290842.
1
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Additional Supporting Information may be found online
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[
Journal of Heterocyclic Chemistry
DOI 10.1002/jhet