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1
0 lg soluble protein.
CAD assays were carried out essentially as described by
Wyrambik and Grisebach (1975). Assays consisted of
00 lM coniferyl alcohol, 50 lM NADP and up to 75 lg
microsomal or 25 lg soluble protein in a total volume of
ml 90 mM Tris/HCl buffer (pH 8.8), coniferyl alcohol
was omitted in the reference. Incubation temperature was
1
1
3
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2
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Acknowledgements
We are indebted to Prof. Dr. A.W. Alfermann (D u¨ ssel-
dorf, Germany) for the suspension cultures of L. nodiflo-
rum. The kind gift of b-peltatin from Prof. Dr. M.
Medarde (Salamanca, Spain) is highly appreciated.
Middel, O., Woerdenbag, H.J., Van Uden, W., Van Oeveren, A., Jansen,
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