Organic & Biomolecular Chemistry
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Journal Name
COMMUNICATION
spectroscopy, inductively coupled plasma-mass spectrometry and
potentiometry.15 These methods require expensive equipment,
maintenance and technically trained operators. Thus, the
development of innovative, simple and low-cost methods is always
in demand. Considering the usefulness of these facile recognition
methods, we further investigated the practical application of probe 3
as an optical solid sensor16 for Hg2+ and Cu2+.
For this investigation, we used TLC plates coated with
Spectrochem GF254 silica-gel, filter paper strips, and 100-200 mesh
silica-gel. The TLC plates and filter paper strips were dipped in a
2M solution of probe 3, dried in air, and subsequently dipped in 10-6
M solutions of Hg2+ and Cu2+ respectively. Immediate color change
was observed, from yellow to dark red for Hg2+ and orange for Cu2+.
Similarly, a solution of probe 3 in THF (15 mL, conc. 2M) was
added to colorless silica gel (100–200 mesh, 2.50 g) and stirred for 1
min and solvent removal afforded a yellow silica-gel. The silica gel
was then treated with a solution of Hg2+ or Cu2+ (2 mL, conc. 10−5M).
An instant color change from yellow to dark red (in the case of Hg2+)
or orange (in the case of Cu2+) was observed. These color changes
were also visible under UV light illumination (Fig. 5). This
experiment indicates that probe 3 has the potential for use as testers
in practical applications such as optical solid sensors for the
detection of Hg2+/Cu2+ in environmental/biological samples.
Acknowledgements
from Key Research Institute Program (NRF201200020209).
DOI: 10.1039/C5OB00907C
Notes and references
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Fig. 5 The variation of color of probe
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We designed and synthesized the new carbazole-pyrimidine
conjugate near-infrared fluorescent probe 3 for the dual detection of
Hg2+ and Cu2+. Probe 3 works as both a colorimetric and ratiometric
fluorescence probe for Hg2+ and Cu2+. It has the capability to detect
Hg2+ and Cu2+ in the nano-molar range with detection limits of 19
and 21 nM respectively. The probe behaves as a naked-eye and
optical solid sensor for the determination of Hg2+ and Cu2+, with
significant color changes on TLC plates, filter paper and silica-gel.
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Thus, the carbazole-pyrimidine conjugate
3
unambiguously
demonstrates the intriguing dual function of Hg2+ and Cu2+ detection
in both the solution and solid states with very low detection limits.
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4 | J. Name., 2012, 00, 1‐3
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