Phytochemistry p. 1751 - 1756 (1988)
Update date:2022-08-17
Topics:
Napolitano, G. E.
Ratnayake, W. M. N.
Ackman, R. G.
The GC properties of 18:5n3 (all-cis-3,6,9,12,15-octadecapentaenoic acid) of algal origin are described for analysis on the popular polyglycol liquid phase Carbowax-20M "bonded" in flexible fused silica open-tubular columns.It is shown with oyster lipids that this fatty acid could be confused with early eluting 20:1 isomers such as the 20:1n11, common in marine animal oils and lipids, and coincides exactly with 20:1n15 of plant origin.Partial hydrazine reduction is demonstrated as a useful technique for confirming the structure of 18:5n3.Key Word Index-Octadecapentaenoic acid; gas-liquid chromatography; phytoplankter fatty acids; marine lipids; hydrazine reduction.
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