Analytical Chemistry p. 229 - 234 (1985)
Update date:2022-08-16
Topics:
Karlsson, Karl-Erik
Wiesler, Donald
Alasandro, Mark
Novotny, Milos
A new reagent, 7-<(chlorocarbonyl)methoxy>-4-methylcoumarin, has been synthesized to form fluorescent derivatives for the liquid chromatography of various hydroxy compounds.Reaction conditions were optimized with model hydroxy steroids and prostaglandins.Microcolumns of high chromatographic efficiency were employed to resolve isomeric compounds in both standard and biological mixtures.
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