sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate

Base Information

• Chemical Name: sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate
• CAS No.: 193149-74-5
• Molecular Formula: C20H14 N6 O11 S2 . Na
• Molecular Weight: 600.47
• European Community (EC) Number: 693-016-8
• DSSTox Substance ID: DTXSID30432456
• Mol file: 193149-74-5.mol

Synonyms:2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt;WST-8

Chemical Property of sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate

Chemical Property:

• Appearance/Colour: pale powder
• PSA: 266.62000
• LogP: 4.05220
• Storage Temp.: under inert gas (nitrogen or Argon) at 2-8°C
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 13
• Rotatable Bond Count: 4
• Exact Mass: 599.99814188
• Heavy Atom Count: 40
• Complexity: 1080

Purity/Quality:

99% ^*data from raw suppliers

WST-8 ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]
• description: WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.
• Uses: A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations. WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.