Pullulanase

Base Information

• Chemical Name: Pullulanase
• CAS No.: 9075-68-7
• Molecular Formula: NULL
• Molecular Weight: 0
• Mol file: 9075-68-7.mol

Synonyms:Amylo-1,6-α-glucosidase;Amylopectin 6-glucanohydrolase;Amylopectin-1,6-β-glucohydrolase;Amylux S;Debranching enzyme;E.C.3.2.1.41;E.C. 3.2.1.69;Glucanohydrolase, amylopectin 6-;Glucoamylase P;Kleistasde PL;Limit dextrinase;Optimax 300L;Optimax L 1000;PLL Y-NA;Plunaz;Promozyme;Promozyme 200L;Promozyme 400L;Promozyme 460;Promozyme D;R-enzyme;Starch debranching enzyme;α-Dextrin 6-glucanohydrolase;

Chemical Property of Pullulanase

Chemical Property:

• Appearance/Colour: lyophilized powder
• Vapor Pressure: 0Pa at 25℃
• PSA: 0.00000
• Density: 1.25 g/mL at 25 °C(lit.)
• LogP: 0.00000
• Storage Temp.: 2-8°C
• Water Solubility.: 100g/L at 25℃

Purity/Quality:

99%, ^*data from raw suppliers

Pullulanase microbial ^*data from reagent suppliers

Safty Information:

• Safety Statements: 22-24/25

MSDS Files:

Total 1 MSDS from other Authors

Useful:

• Description: As an an amylolytic exoenzyme, Pullulanase is one of the most essential endo-type enzyme that degrades pullulan. It is found in plants and microorganisms, in which it is synthesized as an extracellular, cell surface-anchored lipoprotein by the Gram-negative bacterium such as Bacillus brevis, genus Klebsiella. Type I pullulanases specifically cleaves α-1,6-glucosidic linkages in polysaccharides, such as starch, amylopectin, glycogen, dextrin, pullulan and related oligosaccharides, whilst type II pullulanases are effective to attack α-1,6-glucosidic linkages. This enzyme is applied on a large scale in glucose and maltose syrup industries, where it is combined with the activities of other enzymes, such as β-amylase and isoamylase to manufacture maltose and oligosaccharides. Besides, it commercially serves as an industrial enzyme preparation in various reactions, such as the production of branched cyclodextrins, where pullulanase can catalyze the condensation reaction. Pullulanase is also applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.
• Uses: One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C. Pullulanase is a glucanase that degrades pullulan. It is produced by the Gram-negative bacterium Klebsiella and is used for starch-debranching as well as to study polysaccharide utilization.