5(6)-CARBOXYFLUORESCEIN DIACETATE

Base Information

• Chemical Name: 5(6)-CARBOXYFLUORESCEIN DIACETATE
• CAS No.: 79955-27-4
• Molecular Formula: C25H16 O9
• Molecular Weight: 920.78
• Hs Code.: 32049010
• Mol file: 79955-27-4.mol

Synonyms:5-Carboxyfluoresceindiacetate

Chemical Property of 5(6)-CARBOXYFLUORESCEIN DIACETATE

Chemical Property:

• Vapor Pressure: 3.61E-20mmHg at 25°C
• Melting Point: >250°C
• Boiling Point: 693.1°C at 760 mmHg
• PKA: 6.4(at 25℃)
• Flash Point: 241.9°C
• PSA: 125.43000
• Density: 1.57g/cm³
• LogP: 3.80340
• Storage Temp.: 2-8°C
• Solubility.: DMSO: soluble

Purity/Quality:

99% ^*data from raw suppliers

5-Carboxyfluorescein diacetate ^*data from reagent suppliers

Safty Information:

• Hazard Codes: T
• Statements: 45-46-23/24/25-36/37/38
• Safety Statements: 53-22-26-36/37/39-45

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Uses: Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. 5-Carboxyfluorescein diacetate is used in the synthesis of Rhodamines. 5-Carboxyfluorescein Diacetate is used in the synthesis of Rhodamines. Dyes and metabolites.

Technology Process of 5(6)-CARBOXYFLUORESCEIN DIACETATE

There total 5 articles about 5(6)-CARBOXYFLUORESCEIN DIACETATE which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 99.0%

acetic anhydride (108-24-7) + 5-carboxyfluoroscein (76823-03-5) → 5-Carboxyfluorescein diacetate (79955-27-4)

Guidance literature:

With sulfuric acid; at 120 ℃;

DOI:10.1002/cbic.201600017

Reference yield:

acetic anhydride (108-24-7) + recorcinol (108-46-3) + trimellitic Anhydride (552-30-7,145253-53-8) → 6-carboxyfluorescein diacetate (3348-03-6) + 5-Carboxyfluorescein diacetate (79955-27-4)

Guidance literature:

recorcinol; trimellitic Anhydride; With zinc dibromide; at 180 ℃; for 1.33333h;

acetic anhydride; for 2h; Overall yield = 4.3 g; Reflux;

DOI:10.1039/c4cc04120h

Reference yield:

recorcinol (108-46-3) → 5-Carboxyfluorescein diacetate (79955-27-4)

Guidance literature:

Multi-step reaction with 2 steps

1: methanesulfonic acid / 12 h / 80 - 85 °C

2: 5.17 g / pyridine / 0.25 h / 85 °C

With pyridine; methanesulfonic acid;

DOI:10.1039/b309196a

upstream raw materials:

acetic anhydride

5-carboxyfluoroscein

recorcinol

trimellitic Anhydride

Downstream raw materials:

5-carboxyfluoroscein

5-carboxyfluorescein

Refernces

• 1、 -. "NEW TECHNOLOGY TO CONJUGATE THE TACCALONOLIDE MICROTUBULE STABILIZERS WITH LINKERS/PAYLOADS". Paragraph 0486; 0487. . Retrieved 2020/09/27.
• 2、 Schulte-Zweckel, Janine,Rosi, Federica,Sreenu, Domalapally,Schr?der, Hendrik,Niemeyer, Christof M.,Triola, Gemma. "Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics". supporting information. p. 12761 - 12764. Retrieved 2015/05/20.