CID 6992709

Base Information

• Chemical Name: CID 6992709
• CAS No.: 5625-37-6
• Molecular Formula: C8H18N2O6S2
• Molecular Weight: 302.373
• Hs Code.: 29335995
• Mol file: 5625-37-6.mol

Synonyms:

Chemical Property of CID 6992709

Chemical Property:

• Appearance/Colour: White/clear crystalline powder
• Melting Point: >300 °C(lit.)
• Refractive Index: 1.6300 (estimate)
• PKA: 6.8(at 25℃)
• PSA: 131.98000
• Density: 1.505 g/cm³
• LogP: 0.41700
• Storage Temp.: Store at RT.
• Solubility.: 0.1 M NaOH: 0.25 g/mL, clear, colorless
• Water Solubility.: Soluble in water.
• XLogP3: -6.9
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 4
• Exact Mass: 302.06062865
• Heavy Atom Count: 18
• Complexity: 370

Purity/Quality:

99% ^*data from raw suppliers

PIPES Free Acid ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi
• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 22-24/25-37/39-26

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: C1C[NH+](CC[NH+]1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-]
• description: PIPES free acid, also known as 1,4-piperazinediethanesulfonic acid, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research.PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH.
• Uses: Piperazine-N, N'-bis- (2-ethanesulphonic acid) ultra -pure >99.5%) is an important chemical intermedia in all kinds of chemical fields. 1,4-Piperazinediethanesulfonic acid, piperazine-N, N’-bis- (2-ethanesulfonic acid) (PIPES) is also one of the zwitterion buffers and the most appropriate buffer for the biological experiments using an intracellular solution since the Pk2 of Pipes is 6.8 at 20 degrees Celsius. A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH. PIPES, is used buffering agent in biochemistry. PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples., PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8. A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.
• Description: PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Technology Process of CID 6992709

There total 2 articles about CID 6992709 which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 86.3%

piperazine (110-85-0) + Na-salt of 2-chloroethane-1-sulfonic acid (15484-44-3) → 2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6)

Guidance literature:

With sodium hydroxide; In water; for 3h; pH=9 - 9.5;

Reference yield:

→ 2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6)

Guidance literature:

Natriumbromaethansulfonat, Piperazin*6H2O;

DOI:10.1021/bi00866a011

upstream raw materials:

piperazine

Na-salt of 2-chloroethane-1-sulfonic acid

Refernces

• 1、 -. "Stabilizing labeled antibody using amino acids". . . Retrieved 2011/11/07.