4-Nitrophenyl phosphate

Base Information

• Chemical Name: 4-Nitrophenyl phosphate
• CAS No.: 330-13-2
• Molecular Formula: C6H6 N O6 P
• Molecular Weight: 219.09
• Hs Code.: 2919900090
• European Community (EC) Number: 206-353-9
• NSC Number: 404086
• UNII: 59NF9TE3BA
• DSSTox Substance ID: DTXSID60861876
• Nikkaji Number: J1.008.477F,J1.008.478D,J1.222.960G,J32.922C
• Wikipedia: Para-Nitrophenylphosphate
• Wikidata: Q3009340
• Pharos Ligand ID: GP3JS8T23HFZ
• Metabolomics Workbench ID: 49791
• ChEMBL ID: CHEMBL24231
• Mol file: 330-13-2.mol

Synonyms:4-nitrophenyl phosphate;4-nitrophenylphosphate;diammonium nitrophenylphosphate;dipotassium nitrophenylphosphate;disodium nitrophenylphosphate;magnesium nitrophenylphosphate;monosodium nitrophenylphosphate;nitrophenylphosphate;p-nitrophenol phosphate;p-nitrophenyl phosphate;sodium nitrophenylphosphate

Chemical Property of 4-Nitrophenyl phosphate

Chemical Property:

• Vapor Pressure: 3.55E-09mmHg at 25°C
• Melting Point: 153 °C
• Boiling Point: 457.8°Cat760mmHg
• PKA: 0.86±0.30(Predicted)
• Flash Point: 230.7°C
• PSA: 295.81000
• Density: 1.712g/cm³
• LogP: -1.68830
• Storage Temp.: 2-8°C
• Solubility.: H2O: 0.1 g/mL, clear
• XLogP3: 0.3
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 2
• Exact Mass: 218.99327391
• Heavy Atom Count: 14
• Complexity: 250

Purity/Quality:

98%min ^*data from raw suppliers

p-Nitrophenyl Phosphate, DiTris Salt Excellent substrate for alkaline phosphatase-based ELISA assays. ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Chemical Classes: Other Classes -> Organophosphates, Other
• Canonical SMILES: C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O
• Uses: 4-Nitrophenyl phosphate undergoes hydrolysis readily, and is used for measuring alkaline phosphatase activity in human serum.

Technology Process of 4-Nitrophenyl phosphate

There total 24 articles about 4-Nitrophenyl phosphate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

sodium bis(4-nitrophenyl) phosphate (4043-96-3) → 4-nitro-phenol (100-02-7,78813-13-5,89830-32-0) + mono-p-nitrophenyl phosphate (330-13-2)

Guidance literature:

With [2,2]bipyridinyl; zinc(II) nitrate; 5E-3M 2,4,6-trimethylpyridine buffer; In water; acetonitrile; at 25 ℃; for 72h; Rate constant; pH 4.8, other reagents;

Reference yield:

Bis(p-nitrophenyl) phosphate (645-15-8) → 4-nitro-phenol (100-02-7,78813-13-5,89830-32-0) + mono-p-nitrophenyl phosphate (330-13-2)

Guidance literature:

With 2,4,6-trimethylpyridine-HNO₃-NaNO₃ buffer pH 6.1; <6,6'-(Me2HNCH2CC)2bpy>(NO3)2; copper(II) nitrate; In ethanol; water; at 20 ℃; Rate constant; Mechanism; var. of ratio ligand:Cu, catalyst, without catalyst;

DOI:10.1039/C39950001205

Reference yield:

[(HO)(NH2(CH2)2NH2)2Co(PO4)Co(NH2(CH2)2NH2)2(O3POC6H4NO2)] (93000-36-3) → 4-nitro-phenol (100-02-7,78813-13-5,89830-32-0) + mono-p-nitrophenyl phosphate (330-13-2) + trans-{Co(en)2(OH)(OPO3)}(1-) + cis-{Co(en)2(OH)(OPO3)}(1-)

Guidance literature:

With OH^(1-); In water; hydrolysis (0.4 M OH(1-)) of aq. soln. of educt at 25.+-.1°C for24 h; monitored by NMR;

DOI:10.1021/ja00337a026

upstream raw materials:

4-nitro-phenol

phosphoric acid monophenyl ester

O-phenyl phosphorodichloridate

4-nitro-phenyl diphenyl phosphate

Downstream raw materials:

phosphoric acid benzyl ester-(4-nitro-phenyl ester)

4-nitro-phenol

p-nitrophenyl sulfate

disodium (4-nitrophenyl)phosphate

Refernces

• 1、 Bencini, Andrea,Berni, Emanuela,Biancini, Antonio,Giorgi, Claudia,Valtancoli, Barbara,Chand, Dillip Kumar,Schneider, Hans-Joerg. "Proton and Cu(II) binding to tren-based tris-macrocycles. Affinity towards nucleic acids and nuclease activity". . p. 793 - 800. Retrieved 2003.
• 2、 Mukaiyama,Hashimoto. "". . p. 196. Retrieved 1971.
• 3、 K?vári, Endre,Kr?mer, Roland. "Rapid phosphodiester hydrolysis by an ammonium-functionalized copper(II) complex. A model for the cooperativity of metal ions and NH-acidic groups in phosphoryl transfer enzymes". . p. 12704 - 12709. Retrieved 1996.
• 4、 -. "P-nitrophenyl phosphate disodium and preparation method thereof". . . Retrieved 2020/01/12.
• 5、 Chu, Yuan,Williams, Nicholas H.,Hengge, Alvan C.. "Transition States and Control of Substrate Preference in the Promiscuous Phosphatase PP1". . p. 3923 - 3933. Retrieved 2017/08/07.