Glycine p-nitroanilide

Base Information

• Chemical Name: Glycine p-nitroanilide
• CAS No.: 1205-88-5
• Molecular Formula: C8H9N3O3
• Molecular Weight: 195.178
• Hs Code.: 2924299090
• DSSTox Substance ID: DTXSID30152884
• Wikidata: Q83019659
• Mol file: 1205-88-5.mol

Synonyms:Glycine p-nitroanilide;1205-88-5;H-Gly-pNA;2-amino-N-(4-nitrophenyl)acetamide;Glycine 4-nitroanilide;GLYCINE-p-NITROANILIDE;Acetamide, 2-amino-N-(4-nitrophenyl)-;N-(4-nitrophenyl)glycinamide;H-Gly-pNA*HCl;SCHEMBL3677488;DTXSID30152884;MFCD00038175;STK377845;AKOS005449218;BS-25051;Glycine p-nitroanilide, protease substrate;FT-0693005;ACETAMIDE,2-AMINO-N-(4-NITROPHENYL)-;J-004367;N-(2-Hydroxyethyl)piperazine-N'-2-hydroxypropane sulfonic acid

Chemical Property of Glycine p-nitroanilide

Chemical Property:

• Vapor Pressure: 2.37E-08mmHg at 25°C
• Refractive Index: 1.654
• Boiling Point: 451.7 °C at 760 mmHg
• Flash Point: 227 °C
• PSA: 100.94000
• Density: 1.413 g/cm³
• LogP: 1.78850
• Storage Temp.: −20°C
• XLogP3: 0.8
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 2
• Exact Mass: 195.06439116
• Heavy Atom Count: 14
• Complexity: 218

Purity/Quality:

98%,99%, ^*data from raw suppliers

Glycine 4-nitroanilide ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: C1=CC(=CC=C1NC(=O)CN)[N+](=O)[O-]
• Uses: Substrate for glycine aminopeptidase. H-Gly-pna acts as proline arylaminidase substrate, for enzyme determination in human serum. Synthesis of some amide derivatives and their biological activity.

Technology Process of Glycine p-nitroanilide

There total 7 articles about Glycine p-nitroanilide which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

Boc-Phe-Gly-NHPh(p-NO2) → N-tert-butoxycarbonyl-L-phenylalanine (13734-34-4) + glycine p-nitroanilide (1205-88-5)

Guidance literature:

With α-chymotrypsin; In water; at 25 ℃; Equilibrium constant; phosphate buffer pH=7;

DOI:10.1016/S0040-4020(01)80374-4

Reference yield:

N-(4-nitrophenyl)-α-<(tert-butoxycarbonyl)amino>acetamide (82518-64-7) → glycine p-nitroanilide (1205-88-5)

Guidance literature:

With hydrogenchloride; In ethyl acetate; at 20 ℃;

DOI:10.1016/j.foodchem.2016.03.097

Reference yield:

2-chloro-N-(4-nitro-phenyl)-acetamide (17329-87-2) → glycine p-nitroanilide (1205-88-5)

Guidance literature:

With ammonium hydroxide;

upstream raw materials:

2-chloro-N-(4-nitro-phenyl)-acetamide

Nα-benzyloxycarbonyl-L-glycyl-p-nitroanilide

4-nitro-aniline

N-(4-nitrophenyl)-α-<(tert-butoxycarbonyl)amino>acetamide

Downstream raw materials:

Boc-Gly-Gly-pNA

((S)-3-Methyl-1-{[(4-nitro-phenylcarbamoyl)-methyl]-carbamoyl}-butyl)-carbamic acid benzyl ester

Boc-L-Asn-Gly-pNA

Boc-L-Gln-Gly-pNA

Refernces

• 1、 Kaneda, Makoto,Yonezawa, Hiroo,Uchikoba, Tetsuya. "Purification and Some Properties of a Protease from the Sarcocarp of Musk Melon Fruit". . p. 2100 - 2102. Retrieved 2007/10/03.
• 2、 Okada,Tsuda,Hirata,Nagamatsu,Okamoto. "Synthesis of chromogenic substrates specific for human spleen fibrinolytic proteinase (SFP) and human leukocyte elastase (LE).". . p. 4060 - 4068. Retrieved 2007/10/02.