Nitroblue tetrazolium chloride

Base Information

• Chemical Name: Nitroblue tetrazolium chloride
• CAS No.: 298-83-9
• Deprecated CAS: 121287-37-4,83800-46-8,67740-22-1,141102-30-9,562102-25-4,679787-05-4,867000-48-4,87714-63-4,1401448-88-1,1422392-93-5,2158197-17-0,1401448-88-1,141102-30-9,562102-25-4,67740-22-1,679787-05-4,83800-46-8,87714-63-4
• Molecular Formula: C₄₀H₃₀N₁₀O₆*2Cl
• Molecular Weight: 817.648
• Hs Code.: 32041900
• European Community (EC) Number: 206-067-4
• UNII: X44P41F7ZK
• DSSTox Substance ID: DTXSID6046719
• Wikipedia: Nitro_blue_tetrazolium_chloride
• Wikidata: Q906440
• NCI Thesaurus Code: C81104
• ChEMBL ID: CHEMBL403063
• Mol file: 298-83-9.mol

Synonyms:Blue, Nitrotetrazolium;Nitro-BT;Nitroblue Tetrazolium;Nitroblue, Tetrazolium;Nitrotetrazolium Blue;Tetrazolium Nitroblue;Tetrazolium, Nitroblue

Chemical Property of Nitroblue tetrazolium chloride

Chemical Property:

• Appearance/Colour: yellow crystals
• Melting Point: 200 °C
• Refractive Index: 1.7350 (estimate)
• PSA: 179.28000
• Density: 1.5521 (rough estimate)
• LogP: 1.29040
• Storage Temp.: 2-8°C
• Sensitive.: Light Sensitive
• Solubility.: methanol: 50 mg/mL, clear, very strongly yellow
• Water Solubility.: Soluble in water, ethanol and methanol.
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 12
• Rotatable Bond Count: 9
• Exact Mass: 816.1726841
• Heavy Atom Count: 58
• Complexity: 1180

Purity/Quality:

99.0% ^*data from raw suppliers

Nitro Blue Tetrazolium Chloride ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xn,Xi
• Hazard Codes: Xn,Xi
• Statements: 22-68/20/21/22-20/21/22-36-36/37/38-41
• Safety Statements: 36-45-36/37-26-39

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Dyes -> Other Dyes
• Canonical SMILES: COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]
• Description: Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.
• Uses: Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Technology Process of Nitroblue tetrazolium chloride

There total 1 articles about Nitroblue tetrazolium chloride which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

Nitroblue formazan (16325-01-2) + isopentyl nitrite (110-46-3) → nitroblue tetrazolium chloride (67740-22-1,298-83-9,87714-63-4)

Guidance literature:

With hydrogenchloride;

DOI:10.1021/ja01604a051 DOI:10.1021/ja01523a070

Reference yield:

cobalt(II) sulphate heptahydrate + nitroblue tetrazolium chloride (67740-22-1,298-83-9,87714-63-4) + 4-(2-pyridylazo) resorcinol → 3,3'-(3,3'-dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium][Co(II)(4-(2-pyridylazo)resorcinolate)2]

Guidance literature:

In chloroform;

DOI:10.2298/JSC140514102V

Reference yield:

nitroblue tetrazolium chloride (67740-22-1,298-83-9,87714-63-4) → nitroblue tetrazolium diformazan

Guidance literature:

With oxygen; thiocolchicoside; sodium chloride; In phosphate buffer; at 28 ℃; pH=7.4; Further Variations:; Reagents; Kinetics; UV-irradiation;

upstream raw materials:

Nitroblue formazan

isopentyl nitrite

Downstream raw materials:

C₄₀H₃₁N₁₀O₆⁽¹⁺⁾*Cl^(1-)