FLUORESCEIN DIPHOSPHATE, TETRAAMMONIUM SALT

Base Information

• Chemical Name: FLUORESCEIN DIPHOSPHATE, TETRAAMMONIUM SALT
• CAS No.: 217305-49-2
• Molecular Formula: C₂₀H₁₄O₁₁P₂*2H₃N
• Molecular Weight: 526.333
• Mol file: 217305-49-2.mol

Synonyms:Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-bis(phosphonooxy)-, tetraammonium salt (9CI); Fluorescein diphosphatetetraammonium salt

Chemical Property of FLUORESCEIN DIPHOSPHATE, TETRAAMMONIUM SALT

Chemical Property:

• PSA: 199.99000
• LogP: 6.45520
• Storage Temp.: -20°C

Purity/Quality:

99% ^*data from raw suppliers

Fluorescein diphosphate tetraammonium salt ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: Fluorogenic alkaline phosphatase probe.

Technology Process of FLUORESCEIN DIPHOSPHATE, TETRAAMMONIUM SALT

There total 2 articles about FLUORESCEIN DIPHOSPHATE, TETRAAMMONIUM SALT which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

fluorescein diphosphate (134869-03-7) → 3',6'-Bis(phosphonooxy)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one diammonium salt (197777-66-5,217305-49-2)

Guidance literature:

With ammonium acetate; In ethanol; at -5 ℃; for 1h; Yield given;

DOI:10.1080/00304949709355232

Reference yield:

3',6'-Bis(bis(benzyloxy)phosphoryloxy)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one (197777-65-4) → 3',6'-Bis(phosphonooxy)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one diammonium salt (197777-66-5,217305-49-2)

Guidance literature:

Multi-step reaction with 2 steps

1: H₂ / 5percent Pd/C / ethanol / 1.5 h / -5 °C

2: NH₄OAc / ethanol / 1 h / -5 °C

With ammonium acetate; hydrogen; palladium on activated charcoal; In ethanol;

DOI:10.1080/00304949709355232

Reference yield:

fluorescein diphosphate (197777-66-5,217305-49-2) → fluorescein free acid (518-45-6)

Guidance literature:

With glycine; magnesium chloride; zinc(II) chloride; alkaline phosphatase; for 1h; Quantum yield; also with prostatic acid phosphatase;

DOI:10.1016/S0960-894X(99)00199-7

upstream raw materials:

fluorescein diphosphate

3',6'-Bis(bis(benzyloxy)phosphoryloxy)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

Downstream raw materials:

fluorescein free acid

Refernces