Detail of "101515-08-6"

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the detection of polymerase chain reaction products containing 7-deazapurine moieties

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the detection of polymerase chain reaction products containing 7-deazapurine moieties. Siegert, C. W.; Jacob, A.; Koester, H. (Faculty of Chemistry, University of Hamburg, Hamburg D-20146, Germany). Analytical Biochemistry, 243(1), 55-65 (English) 1996 Academic. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 9, 73 Double-stranded DNA fragments of different length with and without the incorporation of 7-deazapurine deoxynucleotides have been prepd. via the polymerase chain reaction (PCR) using exo(-) Pyrococcus furiosus DNA polymerase, unmodified primers, and c7-dATP and c7-dGTP as the only purine triphosphates.In this article, certain chemicals are used. Some of their cas registry numbers are 101515-08-6 and 9012-90-2 In spite of the presence of some unmodified purine moieties due to the primers, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry revealed remarkable differences between the modified and unmodified DNA fragments. The incorporation of 7-deazapurine nucleotides resulted in a significant redn. of fragmentation and therefore in increased signal intensities and higher mass resoln. The mol. wts. could be detd. with an accuracy of up to 0.03%. Mass resoln. was sufficient to resolve the (M + H)+ signals of the two single strands of a 7-deazapurine-contg. 99-base-pair DNA duplex. Thus, MALDI-OF mass spectrometry offers a very fast and accurate way to detect and analyze short PCR products sufficient in length for many diagnostic applications without gel electrophoresis and labeling. .

Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing [Erratum to document cited in CA114(23):222795z]

Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing [Erratum to document cited in CA114(23):222795z]. Fernandez-Rachubinski, F.; Eng, B.; Murray, W. W.; Blajchman, M. A.; Rachubinski, R. A. (Dep. Pathol., McMaster Univ., Hamilton, ON L8N 3Z5, Can.). DNA Sequence, 1(6), 427 (English) 1991. CODEN: DNSEES. ISSN: 1042-5179. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 9 An author name, omitted from the original article, has been added.In this experiment, several chemicals are used like 9000-94-6 and 101515-08-6 The error was reflected in the abstr. and index entries. .