Detail of "1032-65-1"

Reference

The influence of deoxycytidine monophosphate (dCMP) on the cytotoxicity of hydroxyurea in the embryonic spinal cord of the mouse

The influence of deoxycytidine monophosphate (dCMP) on the cytotoxicity of hydroxyurea in the embryonic spinal cord of the mouse. Herken, R. (Zent. Anat., Univ. Goettingen, Goettingen D-3400, Fed. Rep. Ger.). Teratology, 30(1), 83-90 (English) 1984. CODEN: TJADAB. ISSN: 0040-3709. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) On day 10 of pregnancy, mice received an i.p. injection of 500 mg/kg hydroxyurea (HU) [127-07-1] or 500 mg/kg HU plus 500, 700, 800, or 900 mg/kg dCMP (I) [1032-65-1]. Three hours after application of the substances, the animals received 5 mCi/g [3H]thymidine i.p. One hour later, the animals were sacrificed and the embryos were removed. Autoradiographs of the embryos served to det. the percentage of necroses, labeled necroses, mitoses, labeled mitoses, and labeled neuroepithelial cells of the spinal cord. Optimal inhibition of HU effects was achieved by application of 700 mg/kg dCMP. The cytotoxic effects of HU on neuroepithelial cells could be partially but not completely inhibited by simultaneous application of dCMP. The duration of HU-induced DNA synthesis inhibition was shortened after simultaneous dCMP application. A strong increase of the labeling index and shortening of the G2-phase duration of neuroepithelial cells after simultaneous application of HU and dCMP was striking as compared to the results obtained from untreated or HU-treated animals.

Degradation of nucleic acids with ozone

Degradation of nucleic acids with ozone. VI. Labilization of the double-helical structure of calf thymus deoxyribonucleic acid. Shinriki, Nariko; Ishizaki, Kozo; Sato, Shoko; Miura, Kazunobu; Sawadaishi, Kazuyuki; Ueda, Tohru (Gov. Ind. Dev. Lab., Sapporo 061-01, Japan). Chem. Pharm. Bull., 32(9), 3636-40 (English) 1984. CODEN: CPBTAL. ISSN: 0009-2363. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The degrdn. of calf thymus DNA (0.54 mg/mL) with O3 contg. O gas (O3 content, 0.1 mg/L; flow rate, 70 mL/min) was examd. The degrdn. rates of the 4 nucleotides in native and heat-denatured DNAs were in the following order: dGMP [902-04-5] x dTMP [365-07-1] > dCMP [1032-65-1] x dAMP [653-63-4] and dTMP > dGMP > dCMP x dAMP, resp. In the case of heat-denatured DNA, the rapid degrdn. of the thymine moiety seems to be due to its relatively weak stacking ability, so that it is more exposed to attack. In intact DNA, the double-helical structure of DNA tends to protect the base moieties from attack by O3. At the early stage of ozonization of native DNA, strand scission did not occur but the degrdn. of several guanine and/or thymine moieties was detected. As the ozonization proceeded, strand scissions of DNA and susceptibility to nuclease [9026-81-7] S1 digestion were obsd. Therefore, a specific O3 denaturation or labilization occurred, causing the double-helical structure to become increasingly loose due to the destruction of guanine and/or thymine moieties, and making the structure more accessible to nuclease S1.