Reference

Rapid determination of antioxidants by the TAS process

Rapid determination of antioxidants by the TAS process. Winkelmann, Wilfried; Montag, Alfred (Ordinariat Lebensmittelchem., Univ. Hamburg, Hamburg, Ger.). Lebensmittelchem. Gerichtl. Chem., 31(5), 87-8 (German) 1977. CODEN: LGCHDT. DOCUMENT TYPE: Journal CA Section: 17 (Foods) Antioxidants of edible fats and oils are volatilized with a TAS oven and applied directly to a slica gel chromatog. plate, and another portion of the sample is dild. 1:1 with Me2CO and applied to the plate, along with stds. The plates are developed with C6H6-HOAc (), and compds. detected with 20% molybdophosphoric acid in abs. EtOH. BHA [25013-16-5] and BHT [128-37-0] could be transferred to the thin-layer plate with the TAS oven, but NDGA [500-38-9] and dodecyl gallate [1166-52-5] and octyl gallate [1034-01-1] were not, and appeared in the Me2CO-dild. spots. Levels of 0.01% antioxidant could be detected in a variety of fats and oils.

Analysis of food antioxidants

Analysis of food antioxidants. 1. Colorimetric determination of BHA. 2. Separation and determination of gallates, BHT, BHA, TBHQ, PA, and ethoxyquin by high-pressure liquid chromatography. Pujol Forn, Marti; Lopez Sabater, Maria Carmen (Dep. Bromatol., Toxicol. Anal. Quim. Apl., Univ. Barcelona, Spain). Circ. Farm., 42(282), 3-23 (Spanish) 1984. CODEN: CIFAA3. ISSN: 0366-6425. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) BHA [25013-16-5] was detd. in fats, oils, and fat-contg. foods by a modification of the method of H. Laszlo and L.R. Dugan (1961), based on chromogenic reaction with the Ehrlich reagent and spectrophotometry at 535 nm. The fats were dissolved in petroleum ether and extd. with EtOH. At 0.75-1.50 mg/15 g fortification levels, the recoveries were 83.5-100.4%. Antioxidants were detd. in fat exts. by reversed-phase high-performance liq. chromatog. on C-18, using acetonitrile-water. The anal. detection lines were: Pr gallate [121-79-9] 275, octyl gallate [1034-01-1] 275, dodecyl gallate [1166-52-5] 275, NDGA [500-38-9] 280, TBHQ [1948-33-0] 290, 3-BHA [121-00-6] 290, BHT 278, ascorbyl palmitate [137-66-6] 245, and ethoxyquin [91-53-2] 360 nm.