Detail of "10465-78-8"

Reference

Sidedness of the inhibitory effects of diamide on sodium and water transport in amphibian skin

Sidedness of the inhibitory effects of diamide on sodium and water transport in amphibian skin. Grosso, A.; De Sousa, R. C. (Sch. Med., Univ. Geneva, Geneva, Switz.). Experientia, 34(5), 593-5 (English) 1978. CODEN: EXPEAM. ISSN: 0014-4754. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Diamide [10465-78-8] added to the external soln. bathing frog skin inhibited hormone-induced increases in Na and water transport, but diamide in the inner soln. caused no inhibition. Dithiothreitol (a classic thiol-reducing agent) could block the effect of diamine (a thiol-oxidizing agent), suggesting that perturbation in the ratio between reduced and oxidized glutathione could be involved. Diamide also inhibited the natriferic action of theophylline and cyclic AMP, implying that diamide influenced the hormonal effects at a step beyond the generation of cyclic AMP.

Influence of diamide on aggregation, cytoskeletal proteins, and arachidonic acid metabolism in human platelets

Influence of diamide on aggregation, cytoskeletal proteins, and arachidonic acid metabolism in human platelets. Caruso, D.; Galli, G.; Till, U.; Spangenberg, P.; Loesche, W.; Paoletti, R. (Inst. Pharmacol. Pharmacog., Univ. Milan, Milan, Italy). Thromb. Res., 36(1), 9-16 (English) 1984. CODEN: THBRAA. ISSN: 0049-3848. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 2, 13 Simultaneous addn. of diamide (DIA) [10465-78-8], a SH-oxidizing agent, and collagen causes a deaggregation of otherwise irreversibly aggregating platelets. Thromboxane B2 (TXB2) [54397-85-2] and 12-HETE [71030-37-0] formation is inhibited depending on the concn. ratio between collagen and DIA. Thus, at 0.25 mM DIA and 20 mg/mL collagen neither TXB2 nor 12-HETE were measurable, but a full scale reversible aggregation is induced. Deaggregation is further attained by adding DIA to collagen-induced aggregates at a time, when max. amplitude has been achieved. Investigation of arachidonic acid (AA) [506-32-1] metabolites under these conditions revealed no influence of DIA on AA metab. Therefore, AA metabolization seems to play a minor role in collagen-induced aggregation and DIA-induced deaggregation. Polymn. of certain cytoskeletal proteins of the platelets, after addn. of DIA, parallels DIA-induced deaggregation. DIA inhibits endogenous AA release, probably by interaction with platelet plasma membrane. DIA seems to inhibit the release of the a-granula protein thrombospondin.