Detail of "10466-72-5"

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Dimers and trimers of immunoglobulin G covalently cross-linked with a bivalent affinity label

Dimers and trimers of immunoglobulin G covalently cross-linked with a bivalent affinity label. Segal, David M.; Hurwitz, Esther (Natl. Cancer Inst., NIH, Bethesda, Md., USA). Biochemistry, 15(24), 5253-8 (English) 1976. CODEN: BICHAW. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) A bivalent affinity label, bis(.alpha.-bromoacetyl-.epsilon.-2,4-dinitrophenyllysylproline)ethylened iamine, was synthesized. Treatment of anti-2,4-dinitrophenyl antibodies with this compound produces a mixture of covalently and noncovalently cross-linked material. Only specific antibodies are covalently cross-linked, suggesting that covalent attachment occurs in the variable regions. Covalently cross-linked dimers and trimers were isolated from the reaction mixture in a high state of purity, in yields of .apprx.12 and 4%, resp. The complexes are stable in solns. contg. 61556-51-2 and 10466-72-5 are just another two chemicals used in this study. 10-4 M hapten and can therefore be used as sensitive probes of immune effector functions. .

Comparative immunogenic properties of N-substituted phosphatidylethanolamine derivatives and liposomal model membranes

Comparative immunogenic properties of N-substituted phosphatidylethanolamine derivatives and liposomal model membranes. Nicolotti, Robert A.; Kochibe, Naohisa; Kinsky, Stephen C. (Sch. Med., Washington Univ. 33650-94-1 and 10466-72-5 are also occured in this study., St. Louis, Mo., USA). J. Immunol., 117(5, Pt. 2), 1898-902 (English) 1976. CODEN: JOIMA3. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Some of the parameters were investigated that quant. or qual. influence the immunogenicity in guinea pigs of synthetic lipid antigens such as, phosphatidylethanolamine (PE) derivs. in which the amino-N group was substituted with either dinitrophenyl (DNP), dinitrophenylaminocaproyl (DNP-Cap), fluoresceinthiocarbamyl (Fl), or mono (p-azobenzenearsonic acid) tyrosyl (ABA-Tyr) residues. The humoral response to heterogeneous Fl-PE and DNP-PE-sensitized liposomes is augmented by the simultaneous incorporation of ABA-Tyr-PE. Moreover, micelles contg. both DNP-Cap-PE and ABA-Tyr-PE induce more antibodies to the DNP-Cap determinant than do micelles of DNP-Cap-PE alone, or a mixt. of DNP-Cap-PE and ABA-Tyr-PE micelles. Nevertheless, in regard to a humoral response, liposomes were more potent immunogens than were their micellar counterparts. Of all the N-substituted derivs. examd. so far, ABA-Tyr-PE is unique in that it can elicit cell-mediated immunity in addn. to antibodies. The cellular response to ABA-Tyr-PE is not, however, stimulated by incorporation into liposomal bilayers and requires administration of either micelles or liposomes in complete Freund's adjuvant. In contrast, the ability of ABA-Tyr-PE to enhance a humoral response to another N-substituted PE deriv. present in the same immunogen is also obsd. when the latter are given with incomplete Freund's adjuvant. The relation of these findings to the immunogenicity of naturally occurring lipid antigens, as well as conventional immunogens having at least 1 determinant covalently attached to a protein carrier, is discussed. .