Reference

Analgesic action of and physical dependence on bulleyaconitine A

Analgesic action of and physical dependence on bulleyaconitine A. Tang, Xican; Lui, Xuejun; Lu, Weihua; Wang, Maode; Li, Ailing (Shanghai Inst. Mater. Med., Acad. Sin., Shanghai, Peop. Rep. China). Yaoxue Xuebao, 21(12), 886-91 (Chinese) 1986.Some commonly used reagents like 107668-79-1 is used in this experiment. CODEN: YHHPAL. ISSN: 0513-4870. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) In mice and rats, s.c. or oral administration of bulleyaconitine A (I) [107668-79-1] was effective against several exptl. pains; the analgesic effect of I was 1.8-3.25, 15.3-65.5, and 108-7195 times that of 3-acetylaconitine, morphine, and aspirin, resp., and was not antagonized by naloxone. Pretreatment with reserpine (3 mg/kg, i.p.) antagonized the analgesic effect of I, and the antagonism was reversed by intracerebroventricular injection of 5-HT or i.p. injection of 5-HTP, suggesting that I-induced analgesia is related to brain 5-HT level. In mice, rats, and monkeys, results indicated that tolerance does not develop to the analgesic effect of I, animals do not become dependent on I, and I does not serve as a narcotic substitute in morphine-addicted animals. I had local anesthetic activity in mice. .

Determination of bulleyaconitine A in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry

Determination of bulleyaconitine A in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry. Weng, Weiyu; Xu, Huinan; Huang, Jianming; Wang, Guoquan; Shen, Teng; Zhang, Jianfang (Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, Peop. Rep. China).Several substances are used for example 107668-79-1 which is its cas registry number. Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences, 816(1-2), 315-320 (English) 2005 Elsevier B.V. CODEN: JCBAAI. ISSN: 1570-0232. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A sensitive and specific high-performance liq. chromatog. (HPLC)-electrospray ionization tandem mass spectrometry (MS-MS) was developed for the detn. of bulleyaconitine A (BLA) in human blood plasma. BLA and internal std. (I.S.) ketoconazole were extd. from the plasma by a liq.-liq. extn. The supernatant was evapd. to complete dryness and reconstituted with acetonitrile contg. 0.1% acetic acid before injecting into an ODS MS column. The gradient mobile phase was composed of a mixt. of acetonitrile (contg. 0.1% acetic acid, vol./vol.) and 0.1% acetic acid aq. soln. eluted at 0.3 mL/min. BLA and I.S. were detd. by multiple reaction monitoring using precursor ? product ion combinations at m/z 644.6 ? 584.3 and 531.2 ? 81.6, resp. Linearity was established for the concn. range of 0.12-6 ng/mL. The recoveries of BLA ranged from 96.93 to 113.9% and the R.S.D. was within 20%. The method is rapid and applicable to the pharmacokinetic studies of BLA in human. .