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Oxidant and antioxidant status of plasma and erythrocyte in the bleomycin-administered rats: The protective role of erdosteine and vitamin E

Oxidant and antioxidant status of plasma and erythrocyte in the bleomycin-administered rats: The protective role of erdosteine and vitamin E. Armutcu, Ferah; Soeguet, Sadik; Guerel, Ahmet; Kart, Levent; Coskun, Oemer (Department of Biochemistry and Clinical Biochemistry, Karaelmas University, Zonguldak, Turk.). THOD, Turk Hematoloji-Onkoloji Dergisi, 14(4), 205-213 (English) 2004 Hacettepe Doktorlar Yayinevi. CODEN: TTHDCG. ISSN: 1301-8825. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Bleomycin (BLM) toxicity is assocd. with lipid peroxidn., which is an autocatalytic mechanism leading to oxidative destruction of cellular membranes, and their destruction can lead to the prodn. of toxic, reactive metabolites and cell death. Therefore, we investigated reactive oxygen species prodn., antioxidant enzyme activities and protective effect of vitamin E and erdosteine in BLM-administrated rats. Thirty-five Sprague-Dawley rats were divided randomly into four groups as untreated control, BLM, BLM+erdosteine and BLM+vitamin E groups. At the. end of the treatment, plasma and erythrocytes were obtained and the levels of thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) were measured. Bleomycin administration resulted in the generation of reactive oxygen species in the blood of rats by decreasing the activities of enzymes SOD, CAT and GSH-Px, while increasing the levels of NO and TBARS, an indicative of lipid peroxidn. Erdosteine and vitamin E treatment prevented the increase in the lipid peroxidn. Erdosteine alone significantly prevented the decrease in SOD, CAT and GSH-Px activities in the erythrocyte and plasma. We suggest that erdosteine is more effective on the prevention of BLM-induced hematotoxicity via antioxidant and free radical scavenger properties than vitamin E at the doses used in the present study. However, further studies at different doses of erdosteine are needed to det. most appropriate doses and to clarify the issue.

Efficient Cleavage of DS DNA by Bleomycin Conjugated via Hexaethylene Glycol Linker to Triplex-Forming Oligonucleotides

Efficient Cleavage of DS DNA by Bleomycin Conjugated via Hexaethylene Glycol Linker to Triplex-Forming Oligonucleotides. Vorobjev, Pavel; Tchaika, Olessia; Zarytova, Valentina (Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russia). Nucleosides, Nucleotides & Nucleic Acids, 23(6 & 7), 1047-1051 (English) 2004 Marcel Dekker, Inc. CODEN: NNNAFY. ISSN: 1525-7770. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) New conjugates of bleomycin A5 with oligonucleotides are synthesized. The bleomycin residue was attached to the 3'- or 5'- terminus of hexadecathymidilate via a hexaethylene glycol phosphate linker. Newly designed conjugates were shown to cleave site-specifically both strands of a dsDNA fragment within a triplex. The max. extent of cleavage for individual strand amts. to 61%.